HIV-1 Gag和Env蛋白特异性ADCC反应与AIDS病程进展的关系  

Relationship of HIV-1 Gag and Env specific ADCC with AIDS progression

作　　者：冯晏萌[1] 梁华[1] 邵一鸣 

机构地区：[1]传染病预防控制国家重点实验室中国疾病预防控制中心性病艾滋病预防控制中心,北京100050

出　　处：《中国艾滋病性病》2014年第6期386-388,共3页Chinese Journal of Aids & STD

基　　金：国家自然科学基金(81020108030,31100126);国家十二五重大专项课题(2012ZX10001008,2012ZX10004501-001-006)~~

摘　　要：目的了解艾滋病病毒I型(HIV-1)Gag和Env蛋白特异性抗体依赖性细胞介导的细胞毒作用(ADCC)反应,与艾滋病(AIDS)疾病进展的相关性。方法采集58份未经抗病毒治疗的、感染时间1年以内的男男性行为人群(MSM)标本,利用HIV-1特异性肽库刺激和流式细胞染色技术进行ADCC检测。结果病毒载量与HIV-1Gag特异性CD+2IFN-γ+细胞占CD-3细胞的比例呈显著的正相关(r=0.2919,P=0.0339);而01_AE亚型感染者的病毒载量与HIV-1Env特异性CD+2CD107a+细胞占CD-3细胞的比例呈显著负相关(r=-0.3454,P=0.0454)。结论在HIV-1感染早期,感染者体内可以产生Gag蛋白特异性的ADCC反应,其中Env蛋白特异性的ADCC反应具有控制疾病进展的作用。Objective To investigate the relationship of HIV-1 Gag and Env specific ADCC with AIDS progression. Methods ADCC was detected in 58 blood samples previously collected from an MSM cohort infected with HIV for less than 1 year without antiretroviral therapy by using the methods of ex vivo peptides/protein stimulation and multi-color flow cytometry. Results The plasma viral loads correlated positively with frequency of HIV-1 Gag specific CD2＋ IFN-y＋ cells （r=0. 2919, P=0. 0339）. And the plasma viral loads of patients infected with HIV-1 clade 01-AE correlated negatively with frequency of HIV-1 Env specific CD＋CD107a＋ cells （r=-0. 3454, P = 0. 0454）. Conclusions At early stage of HIV-1 infection, specific ADCC against different proteins of HIV-1 could be detected within 1 year after infection, but only Env specific ADCC showed potential to inhibit virus replication.

关 键 词：艾滋病病毒 GAG蛋白 Env蛋白 病毒载量 抗体依赖的细胞毒作用 

分 类 号：R373.9[医药卫生—病原生物学]