凉山州2009年HIV-1主要流行区分子流行病学调查  

A molecular epidemiologysurveyof HIV-1 in major epidemic area of Liangshan in 2009

作　　者：冯毅[1] 梁姝[2] 肖林[3] 程春林[1] 魏静[1] 秦光明[2] 阮玉华[1] 邢辉[1] 邵一鸣 何翔[1,4] 

机构地区：[1]中国疾病预防控制中心 性病艾滋病预防控制中心,北京102206 [2]四川省疾病预防控制中心,成都610041 [3]凉山彝族自治州疾病预防控制中心,四川西昌615000 [4]广东省疾病预防控制中心 广东省公共卫生研究院,广州511430

出　　处：《中国艾滋病性病》2014年第6期403-405,共3页Chinese Journal of Aids & STD

基　　金：中国-默沙东艾滋病合作项目~~

摘　　要：目的了解凉山州艾滋病病毒I型(HIV-1)流行毒株的亚型、传播途径和病毒在人群中的分布。方法运用反转录巢式聚合酶链反应(PCR)的方法,扩增2009年凉山州266份血浆样本中HIV的gag基因片段。对获得的核酸序列进行系统进化分析。结果凉山HIV-1的流行毒株为CRF07_BC和CRF08_BC毒株,其中CRF07_BC比例为98.3%,为最主要的流行毒株。266份HIV-1阳性样本中,静脉注射吸毒感染者的比例为63.5%,其次为异性性接触感染者,比例为14.3%。结论凉山HIV-1感染者最主要的感染途径是静脉注射吸毒,最主要的流行毒株是CRF07_BC毒株,具有共同的病毒来源。Objective To investigate the subtypes, distribution and transmission routes of human immunodefi ciency virus type I （HIV-1） strains in Liangshan. Methods Reverse transcription and nested PCR amplification were used to obtain the gag gene fragment sequences of the 266 HIV-1 positive plasma samples of year 2009 in Lian gshan. Phylogenetic tree analysis was conducted with the sequences of the samples. Results The subtypes of HIV- 1 strains in Liangshan were CRF07_BC and CRF08_BC. CRF07_BC was the major epidemic strain, accounting for 98.3%. The proportion of intravenous drug users was 63.5% in the 266 HIV-1 positive subjects under investigation, followed by heterosexuals accounting for 14. 3%. Conclusion The main route of HIV-1 transmission in Liang- shan is intravenous drug injection. CRF07_BC is the major HIV-1 strain, and all strains of CRF07_BC have a common ancestor.

关 键 词：I型艾滋病病毒 分子流行病学 主要流行区 CRF07_BC 

分 类 号：R373.9[医药卫生—病原生物学]