2012年杭州市HIV耐药株传播状况的研究  

A study on HIV drug-resistant transmission in Hangzhou,Zhejiang province in 2012

作　　者：黄晶晶[1] 丁建明[2] 郭志宏[1] 杨介者[1] 程洁[2] 许珂[2] 邢辉[3] 张佳峰[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江310051 [2]杭州市疾病预防控制中心,杭州310021 [3]中国疾病预防控制中心性病艾滋病预防控制中心,北京102206

出　　处：《中国艾滋病性病》2014年第6期406-408,422,共4页Chinese Journal of Aids & STD

基　　金：国家“十二五”重大科技专项课题(2012ZX10001002);浙江省医药卫生科技计划(2013KYB059)~~

摘　　要：目的了解2012年浙江省杭州市新报告的艾滋病病毒(HIV)感染者耐药株的传播水平。方法根据世界卫生组织推荐的HIV耐药警戒线调查方法,收集新报告的HIV感染者的血浆样本,进行基因型耐药检测和耐药株传播水平分析。结果共采集58例样本进行调查,最终成功获得序列51例。根据HIV耐药警戒线调查方法,纳入47例进行耐药分析。按pol区分型,CRF01_AE占66.0%(31/47),CRF07_BC占29.8%(14/47),B亚型占2.1%(1/47),新型重组型CRF01_AE/B/C占2.1%(1/47)。3例样本出现监测性耐药突变,包括蛋白酶区的L90M、I85IV突变以及反转录酶区的T215S,Y188L和K103N突变。结论杭州市处于HIV耐药株中度传播水平,且亚型重组复杂化,突变位点多样化。Objective To study human immunodeficiency virus(HIV)drug-resistant(HIVDR)transmission in Hangzhou,Zhejiang province in 2012.Methods Referring to the guidelines for HIV drug-resistant threshold survey(HIVDR-TS)set by WHO,plasma samples of HIV infected individuals were collected to detect drug-resistant genotype and evaluate the HIV drug-resistant transmission prevalence.Results A total of 58 individuals were sampled for study,among which 51 samples were successfully sequenced and 47 were analyzed for drug-resistance according to the method of HIV drug-resistant threshold survey.Based on the pol sequences,HIV subtypes including CRF01_AE(66.0%),CRF07_BC(29.8%),B(2.1%),novel recombinant form CRF01_AE/B/C(2.1%)were identified.Surveillance drug-resistant mutations including L90M,I85IV from protease gene and T215S,Y188L,K103Nfrom the reverse transcriptase gene were found in three samples.Conclusion HIV drug-resistant transmission was catalogued as moderate epidemic level in Hangzhou,and complicated subtype recombination and diverse drug-resistant mutations were found.

关 键 词：艾滋病病毒 耐药警戒线调查 耐药检测 

分 类 号：R373.9[医药卫生—病原生物学]