Effects of Liuwei Dihuang Granule(六味地黄颗粒)on the Outcomes of In Vitro Fertilization Pre-Embryo Transfer in Infertility Women with Kidney-Yin Deficiency Syndrome and the Proteome Expressions in the Follicular Fluid  

作　　者：连方 吴海萃 孙振高 郭颖 石垒 薛明玥 

机构地区：[1]Integrative Medicine Research Centre of Reproduction and Heredity,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine

出　　处：《Chinese Journal of Integrative Medicine》2014年第7期503-509,共7页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Fund of China(No.81072836);the Natural Science Foundation Project of Shandong Science and Technology Agency(No.2009ZRB14306);and the Special Science and Technology Foundation for Doctoral Subject from Ministry of Education of China(No.20093731110006)

摘　　要：Objective： To observe the effects of Liuwei Dihuang Granule （六味地黄颗粒, LDG） for tonifying Kidney （Shen） on the outcomes of in v/tro fertilization pre-embryo transfer （IVF-ET） of infertility women with Kidneyyin deficiency syndrome and to explore its mechanism by detecting the proteome expression in the follicular fluid. Methods： Sixty-six infertility patients of Kidney-yin deficiency syndrome who would undergo IVF-ET, were randomly assigned to a treatment group and a control group according to a random number table, 33 cases in each group. Another 33 cases of non-Kidney-yin deficiency syndrome was taken as a syndrome-control group. Besides Westem routine therapy, LDG was given 3 menstrual cycles before IVF to the treatment group, and a placebo granule to the control and syndrome-control groups. The scores of Kidney-yin deficiency symptoms （sore waist and knees, dry vagina, dysphoria with feverish sensation in the chest, palms and soles, etc.） were assessed, the number of retrieved oocytes, rates of high quality oocytes and embryos, fertility rate and clinical pregnancy rate were recorded, and the follicular fluid was collected on the day when the ovum was picked up, the differential protein expression was detected using two-dimensional gel electrophoresis, and then, matrix assisted laser desorption ionization time-of flight mass spectrometry （MALDI-TOF-MS） was applied to identify the proteins. Results： The syndrome score in the treatment group decreased significantly from 16.09 ± 2.58 to 8.67 ± 2.13, while it changed insignificantly in the control group, with a significant difference in the lowering score between the two groups （P〈0.05）; the high quality rates of oocytes and embryos and clinical pregnancy rate were all superior in the treatment group to the control group （82.29% vs 78.08%, 76.76% vs 68.79%, 63.64% vs 36.36%, all P〈0.05）. The protein expression map from the follicular fluid showed that compared with the control group, 33 differential protein expresObjective： To observe the effects of Liuwei Dihuang Granule （六味地黄颗粒, LDG） for tonifying Kidney （Shen） on the outcomes of in v/tro fertilization pre-embryo transfer （IVF-ET） of infertility women with Kidneyyin deficiency syndrome and to explore its mechanism by detecting the proteome expression in the follicular fluid. Methods： Sixty-six infertility patients of Kidney-yin deficiency syndrome who would undergo IVF-ET, were randomly assigned to a treatment group and a control group according to a random number table, 33 cases in each group. Another 33 cases of non-Kidney-yin deficiency syndrome was taken as a syndrome-control group. Besides Westem routine therapy, LDG was given 3 menstrual cycles before IVF to the treatment group, and a placebo granule to the control and syndrome-control groups. The scores of Kidney-yin deficiency symptoms （sore waist and knees, dry vagina, dysphoria with feverish sensation in the chest, palms and soles, etc.） were assessed, the number of retrieved oocytes, rates of high quality oocytes and embryos, fertility rate and clinical pregnancy rate were recorded, and the follicular fluid was collected on the day when the ovum was picked up, the differential protein expression was detected using two-dimensional gel electrophoresis, and then, matrix assisted laser desorption ionization time-of flight mass spectrometry （MALDI-TOF-MS） was applied to identify the proteins. Results： The syndrome score in the treatment group decreased significantly from 16.09 ± 2.58 to 8.67 ± 2.13, while it changed insignificantly in the control group, with a significant difference in the lowering score between the two groups （P〈0.05）; the high quality rates of oocytes and embryos and clinical pregnancy rate were all superior in the treatment group to the control group （82.29% vs 78.08%, 76.76% vs 68.79%, 63.64% vs 36.36%, all P〈0.05）. The protein expression map from the follicular fluid showed that compared with the control group, 33 differential protein expres

关 键 词：tonify Kidney Kidney-yin deficiency INFERTILITY Liuwei Dihuang Granule protein expression 

分 类 号：R271.9[医药卫生—中西医结合]