ORMDL3和Ets-1基因在反复喘息患儿外周血中的表达  

Expressions of ORMDL3and Ets-1 in peripheral blood of children with recurrent wheeze

作　　者：金蕊 邱灵芝 袁文霄 庄丽丽 胡毓华 周国平 

机构地区：[1]南京医科大学第一附属医院儿科,江苏省210029

出　　处：《江苏医药》2014年第12期1398-1400,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金青年基金(81300023);国家自然科学基金(81170661);江苏省自然科学基金青年基金(BK20131020);江苏高校优势学科建设工程(JX10231081);教育部博士点基金(20113234110010);江苏省普通高校研究生科研创新计划(CX2211_0714)

摘　　要：目的研究血清类黏蛋白1样蛋白3(ORMDL3)和E26转录因子1(Ets-1)基因在反复喘息患儿外周血中的表达以及两者的相关性。方法采用RT-PCR法检测非过敏性正常对照婴幼儿(A组,24例)和反复喘息患儿(B组,25例)外周血中ORMDL3和Ets-1mRNA表达水平。结果B组外周血ORMDL3和Ets-1mRNA表达水平高于A组(P<0.05);ORMDL3与Ets-1基因表达水平呈正相关(r=0.7704,P<0.05)。结论 ORMDL3和Ets-1基因的表达水平与患儿的反复喘息密切相关。Objective To investigate the expressions and relationship of orosomucoid l-like protein 3（ORMDL3） and E26 transformation specific-1 （Ets-1） in peripheral blood of children with recurrent wheeze. Methods The mRNA expressions of ORMDL3 and Ets-1 in the peripheral blood were detected by RT-PCR in 24 cases of non-allergic controls（group A） and 25 cases of recurrent wheeze（group B）. Results The rnRNA expressions of ORMDL3 and Ets-1 in group B were higher than those in group A （P〈0. 05）. There was a positive correlation between the expressions of ORMDL3 and Ets-1 in both groups （r=0. 7704, P〈0. 05）. Conclusion The gene expressions of ORMDL3 and Ets-1 are closely correlated with the pathogenesis of recurrent wheeze in children.

关 键 词：血清类黏蛋白1样蛋白3 E26转录因子1 反复喘息 

分 类 号：R725[医药卫生—儿科]