PGP9.5及NCAM在阻塞性睡眠呼吸暂停低通气综合征患者软腭末梢神经表达的研究  

Expression of PGP9.5 and NCAM in soft palate of obstructive sleep apnea hypopnea syndrome patients

作　　者：王干[1] 罗伟[1,2] 郝红[1,2] 李桂梅[1,3] 曹晓卉[1,3] 王旭平[1,2] 王佩杰[1,2] 张佳[1,2] 何飞[1,2] 

机构地区：[1]安徽医科大学解放军八一临床学院,南京210002 [2]解放军八一医院耳鼻咽喉头颈外科,南京210002 [3]解放军八一医院病理科,南京210002

出　　处：《安徽医科大学学报》2014年第7期988-991,共4页Acta Universitatis Medicinalis Anhui

基　　金：南京军区“十一五”科研创新课题(编号:08MA05110MA047);南京军区“十二五”科研创新课题联合资助项目(编号:11MA052)

摘　　要：目的研究阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者软腭组织中蛋白基因产物9.5(PGP9.5)和神经细胞黏附因子(NCAM)的表达及末梢神经在软腭各层组织中分布,探讨OSAHS患者软腭末梢神经分布特点及调节的变化。方法选取30例OSAHS患者作为实验组,10例排除OSAHS的单纯慢性扁桃体炎患者作为对照组。通过HE染色检测软腭组织中末梢神经的分布,免疫组化检测软腭组织中PGP9.5和NCAM的表达。结果 1实验组软腭不同层次组织中末梢神经的分布不同,末梢神经主要分布在黏膜下层、腺体、血管周围,肌肉组织周围少量分布;2实验组OSAHS患者软腭组织中PGP9.5及NCAM表达明显高于对照组,差异有统计学意义(P<0.05);3 PGP9.5、NCAM的表达水平与呼吸暂停低通气指数(AHI)呈正相关(r=0.706,P=0.01;r=0.636,P=0.01)。结论 OSAHS患者软腭组织中末梢神经的分布及支配异常,且与病情严重程度密切相关。Objective To study the protein gene product 9. 5 （ PGP 9. 5 ） and NCAM （ neural cell adhesion molecule） expression and the peripheral nerve distribution in soft palate of obstructive sleep apnea hypopnea syn-drom （ OSAHS） patients. Methods 30 patients with OSAHS were selceted as the experimental group while 10 patients diagnosed as the simple chronic tonsillitis excluded OSAHS as control group. Nerve distribution in the tissue of the soft palate was evaluated by HE staining detection. The expressions of PGP9. 5 and NCAM were detected in both groups by immunohistochemistry method. Results ①The distributions of different levels in soft palate nerve were different. The distribution of peripheral nerve mainly distributed in the submucosa, glands, blood vessels, and there was a small amount of distribution around the muscle tissue;②The expression of PGP9 . 5 and NCAM in OS-AHS patients were significantly higher than the control group,which had statistical difference （P〈0. 05）; ③ In OSAHS patients, the expressions of NCAM and PGP9. 5 apnea were positively correlated to apnea hypopnea index, the correction indexs were 0. 706 and 0. 636 respectively（P=0. 01）. Conclusion The results indicate that nerve abnormalities occur in the soft palate of OSAHS patients. The more heavier of OSAHS,the more abnormalities in the soft palate.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 软腭 末梢神经 蛋白基因产物9 5 神经细胞黏附因子 

分 类 号：R766.04[医药卫生—耳鼻咽喉科] R766.3[医药卫生—临床医学]