海南省2010年柯萨奇病毒A组16型VP1区基因特征分析  

Genetic characteristics of Coxsackievirus A 16 VP1 in Hainan province of China in 2010

作　　者：张宝敏[1] 陈海云[2] 檀晓娟[1] 李静 冀天娇[1] 曾祥洁[2] 祝双利[1] 许文波[1] 马焱[2] 王世文[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]海南省疾病预防控制中心 [3]扬州第一人民医院

出　　处：《中华实验和临床病毒学杂志》2014年第3期173-175,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项（项目号2013ZX10004-202,2012ZX10004201）

摘　　要：目的 分析2010年引起海南省手足口病流行柯萨奇病毒A组16型（Coxsackievirus A16,CA16）的分子流行病学特征.方法 从海南省2010年手足口病（hand,foot,and mouth disease,HFMD）标本中分离到56株CA16毒株,用RT-PCR的方法对VP1编码区扩增及核苷酸序列的测定和整理分析,并与CA16各基因型和基因亚型的代表株序列构建亲缘关系进化树.结果 其中34株属于B基因型中的B1a基因亚型,22株属于B1b基因亚型.B1a基因亚型的毒株主要分布于海口市以及海南省西北部相邻的4个县市,B1b基因亚型的毒株主要分布于海口市以及海南省中部、西南部相邻的4个县市.结论 2010年海南省B1a和B1b基因亚型在人群中共循环,两个基因亚型呈现一定区域性分布的特点,提示在海南省CA16至少存在两条大的传播链.在2008年至2010年,海南省手足口病例中CA16感染的比例明显上升,分别为8.05％、5.35％、35.45％.Objective To analyzed the molecular epidemic characteristics of Coxsackievirus A 16 （CA16） which had caused the spread of hand-foot-and-mouth disease （HFMD） in Hainan province during 2010.Methods Totally 56 CA16 strains were isolated from patients with HFMD in Hainan Province during 2010.Reverse transcription polymerase chain reaction （RT-PCR） was used to amplify and sequence the VP1 coding region.These sequences were used together with those of CA16 representative strains to construct the phylogenetic tree.Results And those of 34 strains belonged to genetic subtype B1a of B1 genetic subtype,22 strains belonged to genetic subtype B1b.The B1a genetic subtypes strains were mainly isolated in Haikou City and other four adjacent counties located in the northwest of Hainan Province,and the B1b genetic subtypes strains were mainly isolated in Haikou City and other four adjacent counties located in the middle,southwest of Hainan Province.Conclusion Genetic subtype B1 a and B 1b were prevalentand co-occurring in Hainan during 2010.The distribution of CA16 strains had a certain of geographical characteristics.The results suggested there were at least two large chains of CA16 transmission in Hainan.In 2008-2010,the percentage of CA16 infection in Hainan province from HFMD cases was 8.05%,5.35%,35.45%respectively,which indicates it has increased obviously.

关 键 词：柯萨奇病毒感染 手足口病 基因型 

分 类 号：R373[医药卫生—病原生物学]