原子力显微镜技术研究重组人内皮抑素对内皮细胞的作用  

Study on effect of recombinant endostatin on endothelial cells by atomic force microscope

作　　者：何巍[1] 王群 路慧丽[3] 张晶[3] 韩慧利 

机构地区：[1]长春生物制品研究所有限责任公司,吉林长春130062 [2]长春长生生物科技股份有限公司,吉林长春130103 [3]上海交通大学药学院,上海200240

出　　处：《中国生物制品学杂志》2014年第6期848-851,共4页Chinese Journal of Biologicals

摘　　要：目的 应用原子力显微镜（atomic force microscope,AFM）技术研究重组人内皮抑素（recombinant human endo-statin,rhES）对内皮细胞的作用。方法 采用MTT法检测不同浓度的rhES（0.05～2.4μg/ml）对人脐静脉内皮细胞ECV304增殖活力的影响;分别用0.8和2μg/ml的rhES处理ECV304细胞,应用AFM观察内皮细胞整体形貌的变化,SPI 3800 New DFM动力显微镜观察ECV304细胞表面局部形貌的变化。结果rhES可明显抑制ECV304细胞增殖,且呈剂量效应（P〉0.001）;rhES可降低贴壁的ECV304细胞的厚度,且呈剂量±赖效应,使较光滑的细胞表面变粗糙,产生了一些微小的突起;经rhES处理的ECV304细胞表面结构呈现不规则的变化。结论AFM技术具有样品制备简便和分辨率较高等优点,适合贴壁培养细胞的原位观察。Objective To study the effect of recombinant endostatin （rhES） on endothelial cells by atomic force micro- scope （AFM）. Methods The proliferative activities of human umbilical vein endothelial ECV304 cells treated with rhES at various concentrations （0. 05 - 2. 4 μg / ml） were determined by MTT assay. ECV304 cells were treated with 0. 8 and 2 μg/ml rhES respectively, then observed for overall morphology by AFM, and for local morphology on surface by SPI 3800 New DFM. Results The rhES inhibited the proliferation of ECV304 cells significantly （P 〈 0. 001 ）, and decre- ased the thickness of adherent ECV304 cells, both in dose-dependent modes. The surface of cells after treatment with rhES changed from smooth to rough, on which some small processes were formed. However, the surface structure of ECV304 cells after treatment with rhES showed an irregular change. Conclusion The samples for atomic force micro- scopy were easy to prepare, while the resolving power was high, indicating that the method was suitable for in situ observation of adherent cells.

关 键 词：原子力显微镜 内皮抑素 内皮细胞 

分 类 号：Q-336[生物学]