白藜芦醇通过沉默信息调节因子1途径减轻出血性休克导致的大鼠的心肌损伤恢复心肌收缩功能  

Resveratrol Attenuates Hemorrhagic Shock Induced Cardiac Injury and Restores Cardiac Functions via Silent Information Regulator 1 Pathway in Rat Heart

作　　者：崔华楠[1] 李燕[2] 周自强[1] 王妍军[1] 陈楠[1] 吴明营[1] 

机构地区：[1]首都医科大学附属北京同仁医院心血管疾病诊疗中心,100730 [2]北京军区总医院体检中心,100007

出　　处：《医学研究杂志》2014年第6期45-49,共5页Journal of Medical Research

基　　金：北京市教育委员会科技发展计划面上项目(KM201010025018)

摘　　要：目的研究探讨白藜芦醇在出血性休克导致大鼠心肌损伤中的保护作用及机制。方法复制SD大鼠的失血性休克模型,将SD大鼠分为假手术组(Sham组,n=6),失血性休克模型组(HS组,n=6),白藜芦醇治疗组(RSV组,n=6)和白藜芦醇与沉默信息调节因子抑制剂sirtinol共同处理组(RSV+sirtinol组,n=6)。待模型复制完毕后,监测左心室收缩功能,取血液样本测定血清肌酸激酶(CK)及乳酸脱氢酶(LDH)水平,比较各组心肌酶改变。酶联免疫法测定血清中白介素1-β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的浓度,并测定心肌组织中过氧化物歧化酶(SOD)与过氧化氢(H2O2)的浓度。取大鼠的心肌组织定量测定总三磷酸腺苷(ATP),免疫印迹实验测定sirt1的表达。结果失血性休克造成明显的心功能的损伤,明显增加了血清中炎症因子的表达,减少了心肌组织中SOD的表达,升高了H2O2的含量并减少了心肌组织中ATP含量与sirt1的表达,白藜芦醇干预恢复了休克导致的大鼠心功能的损伤、抑制了炎症因子、逆转了心肌组织中SOD与H2O2的含量并增加了心肌组织中ATP的含量与sirt1的表达,而使用sirt1抑制剂能够抑制白藜芦醇的这些作用。结论白藜芦醇对失血性休克大鼠的心功能有保护作用,其保护作用可能是通过sirt1途径发挥的。Objective To explore the possible beneficial effects and mechanisms of resveratrol on hemorrhagic shock induced rat cardiac injury. Methods SD rats of hemorrhagic shock models were duplicated. The animals were grouped into Sham group （Sham group, n = 6） ; hemorrhagic shock group （ HS group, n = 6） ; resveratrol treatment group （ RSV group, n = 6） ; and resveratrol plus sirtinol treatment group （ RSV ＋ sirtinol group, n = 6）. After the models were duplicated, left ventricular contractility was monitored （ ＋ dP/ dt and -dP/dt） , and blood samples were then obtained. The concentrations of creatine kinase （CK） and lactate dehydrogenase （LDH） and inflammatory factors levels in rat serum of interleukin - 1 β（ IL - 1 β） , interleukin - 6 （ IL - 6） , and tumor necrosis factor - a （ TNF -or） were essayed, respectively. The SOD and H202 levels in rat cardiac tissue were also detected. ATP content and Sirtl expression in cardiac tissue were analyzed. Results Hemorrhagic shock resulted in significantly loss of left ventricular contractility, enhanced inflammatory factors in rat serum, and increased H2O2 content, and decreased serum levels of SOD, ATP and sirtl expression in cardiac tissue. Resveratrol treatment significantly reversed the loss of left ventricular contractility, decreased the elevation of inflammatory factors, and reversed the H2O2and SOD contents in cardiac tissue, and increased ATP and sirtl expression in cardiac tissue. However, treatment with resveratrol and the sirtl inhibitor sirtinol countered those effects of resveratrol mentioned above. Conclusion Resveratrol protected rats from hemorrhagic shock and restored cardiac function, and the protective effects may via the sirtl pathway.

关 键 词：白藜芦醇 失血性休克 沉默信息调节因子1 炎症反应 氧化应激 

分 类 号：R544.16[医药卫生—心血管疾病]