单次不同放射线剂量和时间对Balb/c小鼠耳蜗显微和超微结构的影响  

The influence of the single different radiation dose and time on the microscopic structure and ultrastructure of Balb/c mice

作　　者：杨衬[1] 张威[1] 刘小龙[1] 梁勇[1] 李鹏[1] 张奕[1] 袁亚维[2] 

机构地区：[1]南方医科大学南方医院耳鼻咽喉头颈外科,广州510000 [2]南方医科大学南方医院放射治疗科

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第13期979-982,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省科技计划对外合作项目(No:2010B050700021)

摘　　要：目的:观察单次不同放射剂量及时间对Balb/c小鼠耳蜗显微和超微结构的影响,探讨放射线所致感音神经性聋机制的形态学依据。方法:将16只健康Balb/c小鼠随机分成4组(对照组和3个实验组,每组4只),实验组分别给予8、12、16Gy剂量的放射线照射,在照射后第3、7天处死小鼠,取耳蜗标本后行石蜡包埋、组织切片及苏木精-伊红染色,每组取耳蜗标本行扫描电镜观察。结果:扫描电镜观察发现,对照组内毛细胞及外毛细胞排列整齐,无倒伏、紊乱及缺失;各放射组内毛细胞出现轻微倒伏及排列紊乱,外毛细胞偶有缺失。苏木精-伊红染色结果示各放射组耳蜗内毛细胞、外毛细胞、血管纹细胞及支持细胞在照射后第3、7天与对照组比较未出现明显病理形态学异常。结论:在一次给予≤16Gy放射剂量照射后早期小鼠耳蜗形态仅有轻微改变。Objective：To observe the influence of the single different radiation dose and time on the microscopic structure and ultrastructure of Balb/c Mice.Method：Sixteen four-week-old mice were randomly divided into four groups,control group and three experimental groups,then every experimental group was randomly divided into two groups which were respectively sacrificed on the third and seventh day after exposure to different doses of 8,12,16Gy radiation,then paraffin embedding,tissue section,HE staining and scanning electron microscope,observation were performed after a cochlear specimen collected.Result：Inner hair cells and outer hair cells arranged in order,no lodging,disorder or missing in control group under scanning electron microscope.Slight lodgy or disorder occured in inner cells and outer hair cells were missing sometimes in exposed animals,which meat irrespective of irradiation dose.Conclusion：Slight changes of ultrastructure of Balb/c Mice occured in the early period when given eaqual to or less than 16Gy radiation.

关 键 词：小鼠 放射线 耳蜗 聋 感音神经性 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]