毛冬青中三萜皂苷ilexoside O的制备及抗血栓活性研究  

Preparation of triterpenoid ilexoside O from Ilex pubescens and study on its antithrombotic activity

作　　者：金晶[1] 秦聪怡[2] 周园[2] 曾瑞鑫[2] 赵钟祥[2] 

机构地区：[1]中山大学药学院,广东广州510006 [2]广州中医药大学中药学院,广东广州510006

出　　处：《中草药》2014年第13期1834-1837,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81270054;30901954);广州市珠江科技新星专项(2011J2200047);广东省优秀青年教师培养计划(Yq2013045);广东省自然科学基金资助项目(9451040701003203)

摘　　要：目的从毛冬青Ilex pubescens中分离制备三萜皂苷ilexoside O,并探讨其抗血栓活性。方法综合运用大孔树脂、硅胶和重结晶等分离方法制备化合物,采用核磁、质谱等技术鉴定结构;小鼠和大鼠分别ig给予不同剂量的ilexoside O,通过考察其对小鼠的凝血时间、出血时间和大鼠在三氯化铁诱导下腹主动脉血栓形成量的影响评价其药理活性。结果ilexoside O各剂量组与对照组相比,对小鼠出血时间均有明显影响(P<0.01),ilexoside O低剂量组(25 mg/kg)对小鼠凝血时间无明显影响(P>0.05),但中(50 mg/kg)、高(100 mg/kg)剂量组可以明显延长小鼠的凝血时间(P<0.05);ilexoside O各剂量组均可明显减少FeCl3诱导的大鼠腹主动脉血栓形成量(P<0.01)。结论制备得到的ilexoside O显示出明显抗凝血效果,同时能减少血栓形成,有明显的活血作用。Objective To prepare triterpenoid ilexoside O from the roots of Ilex pubescens and to investigate its antithrombotic activity.Methods Ilexoside O was separated and purified by column chromatography and recrystallization,and was identified by 1H-NMR and 13C-NMR analyses.After ig administration,the pharmacological effects of ilexoside O were evaluated by clotting time（CT）,bleeding time（BT）,and the amount of the abdominal aortic blood clots induced by FeCl3 in rats,respectively.Results In comparison with placebo group,ilexoside O showed the obvious effects on BT（P〈 0.01）.The low-dose group（25 mg/kg） of ilexoside O showed no obvious effect on CT（P〉 0.05）,but the mid-（50 mg/kg） and high-（100 mg/kg） dose groups obviously increased CT of mice（P〈 0.05）;Ilexoside O obviously decreased the amount of the abdominal aortic blood clots in the induction of FeCl3 in rats（P〈 0.01）.Conclusion Ilexoside O shows obvious anticoagulant effect and could decrease the amount of the abdominal aortic blood clots,and its related mechanism is one of the problems which need to be further studied.

关 键 词：毛冬青 三萜皂苷 ilexoside O 抗血栓活性 凝血时间 

分 类 号：R284.1[医药卫生—中药学]