乙型肝炎病毒前S1抗原与血清HBV-DNA及HBVM的相关性分析  

Correlation between hepatitis B virus pre-S1antigen and serum HBV-DNA and HBVM

作　　者：魏娟[1] 王生 周迪[3] 张丽翠[1] 程江[1] 

机构地区：[1]石河子大学第一附属医院检验科,新疆石河子832008 [2]新疆自治区中医医院检验科,新疆乌鲁木齐830000 [3]石河子大学第一附属医院中心实验室,新疆石河子832008

出　　处：《中华医院感染学杂志》2014年第14期3391-3393,共3页Chinese Journal of Nosocomiology

基　　金：新疆维吾尔自治区自然科学基金资助项目(2013211A231)

摘　　要：目的探讨乙型肝炎病毒(HBV)前S1抗原与血清HBV-DNA及HBVM的相关性分析,并探讨HBsAg与HBV-DNA之间的关系,HBsAg与前S1抗原之间的关系及HBV-DNA与前S1抗原之间的关系,为临床提供依据。方法选取2012年9月-2013年7月医院接受诊治的乙型肝炎195例患者HBsAg均为阳性,其中HBV-DNA应用核酸扩增荧光定量检测,HBVM(包括前S1抗原、HBeAg、HBeAb、HBcAb、HBsAg、HBsAb)应用ELISA法检测,选取健康体检者96名作为对照组。结果 195例HBsAg阳性患者的血清标本中HBV-DNA的阳性率最高为75.90%,其次为前S1抗原的阳性率51.28%、HBeAg的阳性率28.21%;前S1抗原与HBsAg、HBV-DNA与HBsAg结果差异均有统计学意义(P<0.05),且HBV-DNA与前S1抗原的阳性检出率均显著高于HBsAg;HBV-DNA与前S1抗原两种检测结果差异均有统计学意义(P<0.05),且HBV-DNA阳性率高于前S1抗原;96例健康者其检测结果表明前S1抗原亦均为阴性。结论乙型肝炎病毒前S1抗原与血清HBV-DNA及HBeAg三者在反映病毒复制水平上具有一定的相关性,目前S1抗原与HBV-DNA具有较高的符合率,可以为HBV感染的监测提供更加全面的分析依据。OBJECTIVE To explore the correlation between the hepatitis B virus pre-S1antigen and serum HBVDNA and HBVM,the relationship between HBsAg and HBV-DNA,and the relationship between HBsAg and pre-S1antigen as well as the relationship between HBV-DNA and pre-S1antigen so as to provide guidance for clinical treatment.METHODS From Sep 2012to Jul 2013,a total of 195patients with hepatitis B,with the HBsAg tested positive,were enrolled in the study,then the HBV-DNA was detected by using nucleic acid amplification fluorescence quantitative assay,and the ELISA was employed to detect the HBVM,including the pre-S1antigen,HBeAg,HBeAb,HBcAb,HBsAg,and HBsAb,choose healthy check-up 96cases as control group.RESULTS Of the 195patients with positive HBsAg,the positive rate of HBV-DNA in the serum specimens was 75.90%,followed by the pre-S1antigen（51.28%）and the HBeAg（28.21%）;there was significant difference between the pre-S1antigen and the HBsAg as well as between the HBV-DNA and the HBsAg（P〈0.05）,the positive rate of HBV-DNA or pre-S1antigen was significantly higher than that of HBsAg,and there was significant difference in the positive rate between the HBV-DNA and the pre-S1antigen（P〈0.05）,and the positive rate of HBV-DNA was significantly higher than that of the pre-S1antigen.The results of test of 96healthy people indicated that the pre-SI antigen was negative.CONCLUSIONThere is certain correlation on the level of viral replication between the pre-S1antigen and serum HBV-DNA and HBeAg,And formerS1antigen and HBV-DNA has a high coineiderce rate,for HBV infection status monitoring to provide more comprehensive analysis basis.

关 键 词：乙型肝炎病毒 前S1抗原 乙型肝炎病毒基因 乙型表面抗原 相关性分析 

分 类 号：R512.62[医药卫生—内科学]