北半球高卢蜜环菌的遗传多样性与分子鉴定  

GENETIC DIVERSITY AND MOLECULAR IDENTIFICATION OF NORTHERN HEMISPHERE SPECIES OF ARMILLARIA GALLICA

作　　者：秦国夫[1] 赵俊[1] 田淑敏[1] JarkkoHantula 

机构地区：[1]国家林业局森林病虫害防治总站,沈阳110034 [2]FinnishForestInstitute

出　　处：《林业科学》2001年第2期61-68,共8页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目!(39770 6 18);国家自然科学青年基金!(395 0 0 0 0 1);国家基金委对外交流与合作项目!(39811130 5 81)资助课

摘　　要：从北美、欧洲和中国收集 2 3个高卢蜜环菌菌株 ,PCR扩增其rDNA的IGS和ITS区域 ,用AluⅠ、HaeⅢ、HinfⅠ和TagⅠ四种限制性内切酶进行酶切 ,同时结合随机扩增微卫星 (RAMS)多态性 ,对北半球的高卢蜜环菌的遗传多样性进行了分析。结果发现了高卢蜜环菌的 6种IGS RFLP图谱类型 ,其中gal(B)和gal(D)是国内外尚无报道的新的IGS RFLP型。RFLP系统树发现该种有明显的大陆之间遗传分化 ,存在中国、欧洲、北美和亚洲 4个亚群体。RAMS分析表明北半球高卢蜜环菌存在亚洲、欧洲、北美 中国和北美 欧洲等 4个高卢蜜环菌发育系 ,而且不同大陆的高卢蜜环菌具有较近的亲缘关系 ,而相同大陆的却具有不同的起源 ,特别是北美的高卢蜜环菌两个发育系异源性很高 ,北美东部的发育系同欧洲的关系比较近 ,而北美西部的同亚洲关系比较近。The IGS of 23 isolates of Armillaria gallica from three continentals of Northern Hemisphere were amplified with primers LR12R and O 1,and digested by AluⅠ,Hae Ⅲ,Hinfl,TagⅠ restriction enzyme,respectively.six RFLP patterns were observed,the patterns of gal(A) and gal(B) were only seen in Chinese isolates,gal(C) was occurred around the northern hemisphere,including USA,Canada,Germany,Poland,Luxembourg and China,gal(D) pattern was seen in a European isolate,gal(E) was only found in American isolates.Based on the parsimony tree of RFLP results,four A.gallica sub\|populations were found from these isolates and named the Chinese(CH),European(EU),North American(NA),and Asian(AS) sub\|populations.The Random amplified microsatellites (RAMS) experiments were carried out with primer TCG,CGA,M13,and GAAA,respectively.Based upon the parsimony tree of RAMS results,these isolates were separated into four clades,i.e.the European (EU),Chinese (CH),North American\|Chinese (NACH),and North American\|European (NA\|EU) geographical lineage.Two groups emerged in the NA isolates of A.gallica, and they are more close to EU and CH respective than between.The research suggested that the RAMS are quite effective way for analysis of genetic diversity of fungi.

关 键 词：高卢蜜环菌 遗传多样性 IGS-RFLP RAMS分析 遗传分化 林木病原菌 

分 类 号：S763.150.2[农业科学—森林保护学]