罗氏沼虾脑促性腺激素的初步分离及活性检测  

Partial isolation and bioassay of gonad stimulating hormone from the brain of Macrobrachium rosenbergii

作　　者：廖家遗[1] 张艳[1] 孙继贤[1] 林远声[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《水产学报》2001年第1期5-10,共6页Journal of Fisheries of China

基　　金：国家自然科学基金资助项目! (396 70 10 3)

摘　　要：用SephadexG - 5 0对罗氏沼虾脑抽提液进行了柱层析 ,发现洗脱液中的第二个峰中的 6 3～ 74组分可非常显著地促进体外培养的罗氏沼虾卵母细胞直径增大。进一步检测 ,发现其中仅 6 3～ 6 5组分可显著地促进体外培养的罗氏沼虾卵巢的总蛋白合成 ,进行日本沼虾肌肉注射可显著地促进其卵巢发育。 6 3组分的分子量在7kD以下。结果表明 。The extracts of brain of Macrobrachium rosenbergii were applied on a Sephadex G-50 column (1.6cm× 50cm). Two large peaks of protein, peakⅠ and peakⅡ, were obtained. The Ⅱ C (63-74 fractions) of peakⅡ were able to increase oocyte diameter of M. rosenbergii in vitro very significantly. The 63-65 fractions from Ⅱ C of peakⅡ significantly accelerated the total protein synthesis of the prawn ovary in vitro. By injecting the 63- 65 fractions into muscle of M. nipponense the ovary maturation was promoted significantly after 15 days. The molecular weight of the protein in the fraction 63 was below 7kD. The results show that the gonad stimulating hormone is present in the brain of M. rosenbergii.

关 键 词：罗氏沼虾 脑 保性腺激素 分离 生物活性 检测 

分 类 号：Q959.223.5[生物学—动物学] Q575.12