用免疫金技术检测猪繁殖与呼吸综合征病毒  

A dot immuno gold filtration assay for the detection of porcine reproductive and respiratory syndrome virus

作　　者：李军[1] 林继煌[1] 姜平[2] 何孔旺[1] 倪艳秀[1] 还红华[1] 

机构地区：[1]江苏省农科院农业部畜禽疫病诊断重点开放实验室 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《畜牧与兽医》2001年第2期1-2,共2页Animal Husbandry & Veterinary Medicine

摘　　要：用胶体金标记提纯后的兔抗猪繁殖与呼吸综合征病毒 (PRRSV)IgG ,建立了一种以微孔滤膜为固相载体 ,以红色胶体金为标记物的检测PRRSV的斑点免疫金渗滤法 (DIGFA)。特异性阻断试验与交叉试验证明DIGFA检测PRRSV有较高的特异性。检测 1 5份临床样本 ,其中 3份DIG FA及细胞培养均为阳性 。A dot immuno gold filtration assay(DIGFA)for porcine reproductive and respiratory syndrome virus(PRRSV)was developed.Rabbit anti PRRSV IgG was obtained from the rabbit immunized with the purified PRRSV and confugated to immunogold.15 clinical samples were detected,3 of which were positive both in DIGFA and cell culture,and the virus particles of PRRSV could be seen by EM.It showed that the DIGFA is a sensitive,specific,simple and rapid assay for detecting PRRSV.

关 键 词：猪 繁殖呼吸综合征 斑点免疫金渗滤试验 繁殖-呼吸综合征病毒 检测 

分 类 号：S858.285.3[农业科学—临床兽医学] S852.65[农业科学—兽医学]