rh-bFGF抑制顺铂诱导的人胃腺癌BGC-823细胞凋亡  

The inhibitson of cisplatin-induced apoptosis in BGC-823 human stomach cancer cells by basic fibroblast growth factor

作　　者：辛洪启[1] 袁守军[2] 汤仲明[2] 林剑[1] 洪岸[1] 

机构地区：[1]暨南大学生物工程研究所,广东广州510632 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《暨南大学学报（自然科学与医学版）》2001年第1期120-124,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家"九五"重点攻关资助!项目 (96 -C0 2 - 0 1- 0 3)

摘　　要：目的 :评价外源加入的rh -bFGF对抗肿瘤药物顺铂所致人胃腺癌细胞凋亡的影响 方法 :利用顺铂诱导人胃腺癌BGC - 82 3细胞产生的凋亡作模型 ,体外培养时在顺铂作用前或作用后加入不同浓度的rh -bFGF ,观察细胞形态的变化和细胞凋亡比率的变化 结果 :预培养时rh -bFGF质量浓度为 10ng/mL及 1ng/mL时抑制细胞凋亡的发生 ;共培养时则在质量浓度为 1ng/mL时产生抑制凋亡的作用 结论 :外源加入的rh -bFGF在一定的浓度范围内 ,不论预培养还是与顺铂的共培养 12h ,均可不同程度的抑制顺铂诱导的人胃腺癌BGC - 82 3细胞的凋亡 ,并且这种剂量效应与rh -bFGF影响BGC - 82Aim: To evaluate the suppression of the apoptosis induced by cisplatin in BGC-823 human stomach cancer cells by rh-bFGF. Methods: Using the apoptosis model in BGC-823 human stomach cancer cells cisplatin induced, we studied the change of cytomorphology and the rate of apoptosis cell when pre-cultue or co-culture with recombined human basic fibroblast growth factor(rh-bFGF) in vitro . Results: rh-bFGF can inhibit cisplatin-induced apoptosis in BGC-823 human stomach cancer cells either pre-culture in 12 hours at 1 ng/mL and 10 ng/mL, or co-culture with cisplatin in 12 hours at 1ng/mL. Conclusion: rh-bFGF can inhibit the apoptosis in BGC-823 human stomach cancer cells induced by cisplatin at some concentration range in vitro , it maybe associate with the effect that rh-bFGF influence the cell cycle. [

关 键 词：重组人碱性成纤维细胞生长因子 RH-BFGF 顺铂 人胃腺癌 BGC-823细胞 细胞凋亡 药物抗性 

分 类 号：R979.1[医药卫生—药品] R966[医药卫生—药学]