氧化型染发剂对大鼠皮肤角朊细胞毒作用的实验研究  

Cytotoxicity of Oxidation Hair Dyes on Rat's Keratinocytes.

作　　者：吕冬梅[1] 浦跃朴[1] 尹立红[1] 李云晖[1] 戴修道[2] 杨薇[2] 周德灏[2] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,南京210009 [2]上海医科大学环境卫生教研室

出　　处：《环境与健康杂志》2001年第1期17-19,共3页Journal of Environment and Health

基　　金：卫生部科技基金!资助 (96- 1 - 1 92 )

摘　　要：目的　探讨氧化型染发剂引起皮肤损伤的机制。方法　应用大鼠皮肤角朊细胞的原代培养技术并结合 MTT比色法检测了氧化型染发剂及其两种主要成分——双氧水 (H2 O2 )、对苯二胺 (PPD)及二者的混合物对大鼠皮肤角朊细胞的增殖能力的影响。结果　除 PPD的最低剂量短时间染毒组在一定程度上可刺激细胞增殖外 ,受试样品对大鼠角朊细胞的生长均有不同程度的抑制作用 ,随着染毒剂量的升高和染毒时间的延长 ,OD值逐渐降低 ,染毒剂量与细胞生存抑制率呈剂量 -反应关系 (2 4h染毒后的 r值分别是 :r H2 O2 =0 .981,r PPD=0 .949,r PPD- H2 O2 =0 .96 3,r染发剂 =0 .887)。结论　氧化型染发剂的皮肤损伤作用可能与其细胞毒性有关。Objective To explore the mechanism of the skin lesion induced by oxidation hair dyes Methods The effects of subjects,such as oxidation hair dyes,hydrogen peroxide (H 2O 2) and p phenylenediamine (PPD) and their mixture which were main components of oxidation hair dyes,on the proliferation of rat's keratinocytes were detected by primary culture technique of rat's keratinocytes and MTT colorimetric assay Results All of the observed groups presented a certain inhibition on proliferation of the rat's keratinocytes,except the group exposed to PPD at the lowest dose for the shorter period The OD values decreased with the increase of exposure levels and periods Dose response relationship was observed between the exposure levels and inhibition rates of cell survival(the r values after 24 h exposue: r H 2O 2 =0 981, r PPD =0 949, r PPD-H 2O 2 =0 963, r hair dyes =0 887) Conclusion The skin lesion induced by oxidation hair dyes might associated with its cytotoxicity

关 键 词：氧化型染发剂 细胞毒性 皮肤损伤 角朊细胞 

分 类 号：R996[医药卫生—毒理学] TQ658.34[医药卫生—药学]