叶枯灵在大鼠尿内的代谢产物分析  

Analysis on the metabolites of yekuling in rat urine

作　　者：王宇[1] 李正化[2] 高宁 

机构地区：[1]华西医科大学制药厂 [2]华西医科大学药学院新药设计研究室 [3]公共卫生学院环境卫生研究室,成都610041

出　　处：《中国药理学与毒理学杂志》1992年第4期301-304,共4页Chinese Journal of Pharmacology and Toxicology

基　　金："七.五"国家重点科技攻关项目

摘　　要：大鼠ig叶枯灵后,用HPLC和TLC分离纯化尿提取液中的叶枯灵及其代谢产物,得到六个组分,经MS和UV初步鉴定为叶枯灵,苯甲酸,马尿酸和三个新化合物:肼叉-1.3-二噻茂烷,乙酰肼叉-1.3-二噻茂烷和丙酮酸缩肼叉-1.3-二噻茂烷,通过对代谢物的化学合成,进一步确证了它们的化学结构。Benzole acid l,3-dithiolan-2-ylidenehydrazide (yekuling) is a new sulfurorganic fungicide. The metabolites of yekuling were studied in the rat urine. After the oral administration of yekuling to rats, urine samples were collected in 24 h. Six components, (Ⅰ), (Ⅱ), (Ⅲ), (Ⅳ), (Ⅴ) and (Ⅵ) were isolated and purified from the rat urine by the HPLC and TLC methods. They were considered as 1.3-dithiolan 2-ylidenehydrazide (Ⅰ), acetic acid 1.3-dithiolan2-ylidenehydrazide (Ⅱ ), N-benzoylglycine (Ⅲ ), pyruvic acid 1.3-dithiolan-2-ylidenehydrazone (Ⅳ), the parent drug (Ⅴ) and benzoic acid (Ⅵ). They were confirmed by means of the identification of the chemical synthetic compounds. The metabolic pathway of yekuling was discussed.

关 键 词：叶枯灵 分析 高产液相色谱 

分 类 号：R991[医药卫生—毒理学]