肺巨噬细胞的灌洗动力学研究  

Study on the Lavaging Kinetics of Alveolar Macrophages

作　　者：胡连平[1] 龚诒芬[1] 费丽华[2] 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所 [2]解放军总医院单克隆研究室

出　　处：《卫生毒理学杂志》1990年第1期10-13,共4页Journal of Health Toxicology

摘　　要：用0.02%EDTA.PBS液10ml灌洗全肺10次可获得7.3±0.9×10^7个游离细胞，其洗出细胞的动态变化为：第3次洗出量最多，然后逐次下降，第5次到第10次洗出量基本不变：洗出的各类细胞构成比不随洗肺次数而改变，同样方法改用2m1EDTA.PBS液灌洗，则只洗出2.5±0.3×10^6个游离细胞，其洗出的细胞动态变化为：第3次为洗出高峰，然后依次下降，网状细胞份额随灌洗次数依次升高，“成熟”AM的份额则依次下降，大淋巴样细胞不随灌洗次数而改变。The influence factors on alveolar macrophage ( AM ) adherence and lavaging kinetics of AM were studied in this experiment. The results showed that; (l)a fit temperature and furnish of glucose and Ca and Mg ions would play an important role in AM adherence) ( 2 ) after lungs being lavaged for 10 times with 10 ml 0.02% EDTA· PBS, free cells of 7.3±0.933107 were obtai ned; with 2 ml 0,02% EDTA ·PBS, free cells of 2.5 ± 0. 3 × 106; ( 3 ) in the 10 ml experiment and the 2 ml experiment, the variance of lavaged free cells were occurred in the first three times; maximum number of lavaged free cells appeared at the 3ed time in both experiments; the pro portion of reticulate cells increased and'mature' AM decreased gradually with lavaging in the 2 ml experiment and the proportion of various cells were not changed in the 10 ml experiment.

关 键 词：肺 游离细胞 灌洗动力学 

分 类 号：R99[医药卫生—毒理学]