分泌性中耳炎渗出液的细胞因子检测  

Cytokine expression in effusions of secretory otitis media

作　　者：高为华[1] 刘世援[1] 郝红军[2] 

机构地区：[1]北京大学第一医院耳鼻咽喉科,北京100034 [2]北京大学第一医院神经内科

出　　处：《临床耳鼻咽喉科杂志》2001年第4期157-158,共2页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨分泌性中耳炎渗出液中细胞因子的水平及其炎症的调节机制。方法 :用 EL ISA法检测本病患耳的渗出液中肿瘤坏死因子 -α(TNF-α)和可溶性肿瘤坏死因子受体 (TNFsol R )水平 ,分析其与临床指标间的关系。结果 :渗出液中 TNF- α和 TNFsol R 的检出率均为 10 0 % ;TNF- α值为 (3.42± 2 .2 5 ) ng/ g总蛋白 ,TNFsol R 为 (4 0 5 .80± 2 16 .5 3) ng/ g总蛋白 ,TNFsol R 指数为 (15 4.18± 90 .45 ) U;多次发病的患者 TNFsol R 和 TNFsol R 指数均降低 (P <0 .0 5 )。结论 :细胞因子 TNF- α和 TNFsol R 存在于所有分泌性中耳炎渗出液中 ,是此炎症反应的重要递质。复发的分泌性中耳炎渗出液中 TNFsol R 和 TNFsol R 指数明显降低 (P <0 .0 5 ) ,而 TNF- α无明显变化。Objective:To assess the cytokine level in effusions of secretary otitis media and it's relationship with clinical index.To further study the inflammatory mechanism of otitis media with effusion.Method:The concentration of TNF α and TNFsolRⅡ was quantitatively determined using enzyme linked immunosorbent assay and it was correlated with clinical index in otitis media with effusion using stepwise regression analysins.Result:TNF α and TNFsolRⅡ was found in 100% effusions.Mean values of TNF α were ( 3.42 ± 2.25 )ng/g total protein.Mean values of TNFsolRⅡ were ( 405.80 ± 216.53 )ng/g total protein.Mean values of TNFsolRⅡ index was ( 154.18 ± 90.45 )U.There was a significant decrease of TNFsolRⅡ and TNFsolRⅡ index in patients who recurred (P< 0.05 ).Conclusion:Cytokine TNF α and TNFsolRⅡ are important inflammatory medium which exist in all otitis media effusions.The decrease of the level of TNFsolRⅡ and TNFsolRⅡ index present significantly in middle ear effusions with recurrence,but there is no any relation of TNF α in middle ear effusions with recurrence.

关 键 词：分泌性中耳炎 肿瘤坏死因子-Α 可溶性肿瘤坏死因子受体 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]