重复性注射镉金属硫蛋白诱导小鼠肾脏金属硫蛋白基因的表达  

Metallothionein gene expression in mice kidney induced by multiple short interval injections of cadmium metallothionein

作　　者：逄兵[1] 金泰廙[1] 胡云平[1] 蒋学之[1] Nordberg Gunnar 

机构地区：[1]复旦大学医学院劳动卫生学教研室,上海200032 [2]瑞典UMEA大学环境医学系

出　　处：《中华劳动卫生职业病杂志》2001年第1期40-42,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：欧共体科学研究基金资助项目!(ERB 35 14PL9714 30);瑞典STINT基金资助项目!(STINT UMEA1998)

摘　　要：目的　探讨重复性注射镉金属硫蛋白 (CdMT)对小鼠肾脏金属硫蛋白 (MT)基因表达的影响。方法　以 β actin为内参照 ,采用半定量RT PCR方法检测重复性CdMT染毒后小鼠肾脏MT1和MT2基因的表达水平及与染镉剂量和肾皮质镉含量之间的相关性。结果　随着CdMT剂量的增加 ,MT1、MT2基因的表达水平都相应升高 ,当CdMT染毒总剂量达到 0 .6 0mg/kg时 ,肾皮质中MT1、MT2基因的表达水平分别为 1.6 0± 0 .12及 1.44± 0 .0 5 ,与相应的对照组 (1.2 2± 0 .16及 0 .92± 0 .12 )相比 ,均明显升高 ,差异有显著性 (P <0 .0 5 ,P <0 .0 1) ;肾皮质中MT1及MT2基因表达水平分别与肾皮质中镉的含量呈正相关 ,其相关系数分别为r=0 .72 (P <0 .0 1)及r=0 .71(P <0 .0 1)。结论　镉对MT1及MT2基因表达具有较强的诱导能力 ;Objective To explore the changes of metallothionein(MT) gene expression induced by multiple short interval injections of cadmium metallothionein(CdMT) in mice. Methods With semi quantitative RT PCR,gene expression of MT1 and MT2 in renal cortex were studied following an initial CdMT dose and four subsequent doses administered subcutaneously at an interval of 2 hrs. Results MT1 and MT2 gene expression in renal cortex increased with the increasing of CdMT dose.At the dose of 0.60 mg/kg,MT1 and MT2 gene expression in renal cortex(1.60±0.12 and 1.44±0.05,respectively) were significantly higher than those of controls (1.22± 0.16 and 0.92± 0.12, P <0.05, P <0.01 respectively).MT1 and MT2 gene expression in renal cortex were correlated to Cd level in renal cortex( r =0.72 and r =0.71 respectively, P <0.01). Conclusion Both MT1 and MT2 gene expression in renal cortex could be obviously induced by cadmium.There were similar changing tendencies between MT1 and MT2 gene expression in renal cortex.

关 键 词：镉金属硫蛋白 MT基因表达 RT-PCR 重复性注射 肾 镉 肾毒性 

分 类 号：R135[医药卫生—劳动卫生]