二甲基甲酰胺对大鼠肾脏线粒体和微粒体^(45)Ca转运的影响  

The effect of dimethylformamide on ^(45)Ca transport in rat kidney mitochondria and microsomes in vitro

作　　者：菅向东[1] 阚宝甜[1] 张秀莲[1] 

机构地区：[1]山东医科大学附属医院职业病科,济南250012

出　　处：《中华劳动卫生职业病杂志》2001年第1期62-64,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　研究二甲基甲酰胺 (DMF)对大鼠肾脏线粒体和微粒体4 5Ca转运的影响 ,探讨其肾脏损害的作用机制。方法　采用超速冷冻离心技术分离大鼠肾脏线粒体及微粒体 ,应用放射性同位素技术测定DMF对线粒体和微粒体4 5Ca主动摄取及释放的影响。结果　 (1) 45Ca的摄取 :孵育 10min时 ,DMF 130、195 μmol/L组大鼠肾线粒体4 5Ca摄取量分别为 (4 .2 0± 0 .2 9)、(3.81± 0 .42 )nmol/mgpro,对照组为(5 .18± 0 .40 )nmol/mgpro;微粒体4 5Ca摄取量分别为 (2 .10± 0 .42 )、(2 .0 1± 0 .38)nmol/mgpro ,对照组为(2 .96± 0 .38)nmol/mgpro。DMF两个剂量组与对照组的差异均有显著性 (P <0 .0 5或P <0 .0 1)。 (2 ) 45Ca的释放 :孵育 2min时 ,DMF 6 5、130、195 μmol/L组大鼠肾线粒体4 5Ca残留量分别为 (5 .40± 0 .39)、(5 .0 3±0 .32 )、(4 .70± 0 .5 0 )nmol/mgpro,对照组为 (6 .0 1± 0 .40 )nmol/mgpro ;微粒体4 5Ca残留量分别为 (3.43±0 .2 6 )、(3.18± 0 .31)、(3.0 6± 0 .2 7)nmol/mgpro ,对照组为 (4 .0 6± 0 .32 )nmol/mgpro。DMF两个剂量组与对照组的差异均有显著性 (P <0 .0 1)。(3)DMF与鼠肾线粒体和微粒体4 5Ca的摄取、释放均存在剂量 -效应关系 (前者 :r=0 .918,r=0 .895 ;后者 :r=0 .Objective To observe the influence of dimethylformamide(DMF) on 45 Ca transport in rat kidney mictochondria and microsomes. Methods Mitochondria and microsomes were isolated by ultracentrifugation.Radioactive isotope technique was used to examine the effects of DMF on 45 Ca uptake and release of mitochondria and microsomes and to explore the mechanism of renal injury. Results (1)The 45 Ca uptake of mitochondria in DMF groups(130 μmol/L,195 μmol/L) after 10 min incubation were (4.20±0.29)nmol/mg pro and (3.81± 0.42) nmol/mg pro respectively( P <0.05 or P <0.01),compared with control group (5.18±0.40)nmol/mg pro,and the 45 Ca uptake of microsomes in DMF groups(130 μmol/L,195 μmol/L) after 10 min incubation were (2.10± 0.42)nmol/mg pro,(2.01±0.38)nmol/mg pro respectively( P <0.05 or P <0.01),compared with control group (2.96±0.38)nmol/mg pro.The same results were gained after 20 min and 30 min incubation.(2)The 45 Ca remain in mitochondria of DMF groups(65 μmol/L,130 μmol/L,195 μmol/L) after 2 min incubation were (5.40±0.39)nmol/mg pro,(5.03± 0.32) nmol/mg pro and (4.70±0.50)nmol/mg pro respectively( P < 0.01) ,compared with control group[(6.01±0.40)nmol/mg pro].The 45 Ca remain in microsomes of DMF groups(65 μmol/L,130 μmol/L,195 μmol/L) after 2 min incubation were (3.43± 0.26) nmol/mg pro,(3.18±0.31) nmol/mg pro and (3.06±0.27)nmol/mg pro respectively( P < 0.01) ,compared with control group (4.06± 0.32) nmol/mg pro,indicating increased release of calcium.The same results were gained 4 min and 8 min after incubation.There were dose effect relationships between DMF and 45 Ca uptake or 45 Ca release in both mitochondria and microsomes( r =0.918,0.895,0.886,0.846 respectively). Conclusion DMF may induce disturbance of calcium homeostasis in rat kidney.

关 键 词：二甲基甲酰胺 肾脏 钙稳态 线粒体 微粒体 肾毒性 

分 类 号：R135[医药卫生—劳动卫生]