日本血吸虫感染对小鼠肝药物代谢酶的影响  

EFFECT OF SCHISTOSOMA JAPONICUM INFECTION ON LIVER DRUG-METABOLIZING ENZYMES OF MICE

作　　者：王捷[1] 邱则吟 谢婉[1] 吴幼瑜 

机构地区：[1]四川省医学科学院寄生虫病防治研究所,成都610041

出　　处：《中国寄生虫学与寄生虫病杂志》1993年第1期9-11,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：本文观察了日本血吸虫感染对小鼠肝药物代谢酶的影响。结果,感染20、40和60条血吸虫尾蚴6wk的小鼠,其成巴比妥钠的催眠时间分别为53±9、100±64和172±93min,表明随感染度的增加而明显延长。体外实验观察到各感染度小鼠的肝苯胺羟化酶(Aniline hydroxylase,AH)、氨基比林N-脱甲荃酶(Aminopyrine N-demethylase,APD)的活性和细胞色素P-450(Cy-tochrome P-450)含量均明显降低。即使相同感染度不同病程也显示不同差异,感染30条尾蚴8wk小鼠的肝AH、APD活性和P-450含量比感染4wk小鼠明显降低,分别为对照的32.7％、13.7％和7.7％。说明日本血吸虫感染能明显降低小鼠肝药物代谢酶活性,其影响程度随感染度和病程不同而呈现明显差异。In this paper, the effect of Schistosoma japonicum infection on liver drug-metabolizing enzymes of mice was studied. The prolongation of hypnosis duration of sodium pentobarbital in vivo and the inhibition of liver aniline hydroxylase (AH), aminopyrine N-demethylase (APD) as well as cytochrome P-450(Cyt P-450) in vitro were observed in mice infected with 20,40 and 60 cercariae of S. japonicum after 6 weeks. Meanwhile, with 30 cercariae infection, no significant inhibition of the activity of AH, APD and Cyt P-450 was observed in mice 4 weeks after infection, but significant inhibition of these enzymes was shown by 8 weeks . As compared with control , the activity of AH , APD and Cyt P - 450 decreased to 32. 6%, 13. 7% and 7. 7% at 8 weeks. The results indicate that infection of S. japonicum can inhibit the liver drug-metabolizing capacity of mice, and the degree of inhibition depands on the infectivity and the duration of infection.

关 键 词：血吸虫 肝微粒体 药代动力学 

分 类 号：R532.210.2[医药卫生—内科学]