胰岛素化学发光法测定的建立与评价  

Establishment and evaluation of chemiluminescence immunoassay in the determination of insulin

作　　者：王志刚[1] 徐恋梅[1] 王君耀[2] 毕平安[1] 程巧群[1] 

机构地区：[1]浙江省嘉兴市第二医院,314000 [2]浙江大学医学院附属邵逸夫医院

出　　处：《江西医学检验》2001年第1期17-19,共3页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的 建立化学发光法测定胰岛素及临床糖尿病患者胰岛素治疗跟踪与稳定的可行性评估。方法 CLIA采用一步免疫分析夹心法与RIA的测定结果进行比较。并将该法运用于临床非胰岛素依赖型糖尿病进行观察。结果 CLIA测胰岛素的批内与批间的变异系数分别为4．8％和6．4％，平均回收率为98．6％，与RIA的相关系数为0．8505（P＞0．05）。结论 CLIA法测胰岛素的各技术参数优于RIA法，能及时有效地满足糖尿病患者胰岛素治疗的临床需要。 Objective To establish the chemiluminescence immunoassay CLIA for detection of insulin and to evaluate the possibility of follow－up and stabilization of insulin－treated diabetic patients． Methods A simultaneous one－step immunoenzymatic assay ″sandwich″ for CLIA was compared with radioimmiunoassay RIA． The application of this new technic to non－insulin dependent diabetes mellitus was observed． Results The CVs of within－run and between－run were 4．8％ and 6．4％ respectively． The average rate of recovery was 98．6％． The correlation coefficient with RIA was 0．8505 P＞0．05． Conclusion All the technical parameters of CLIA gain an advantage over those of RIA and can satisfy the clinical requirements of insulin－treated diabetics immediately and effectively． 

关 键 词：胰岛素 化学发光免疫分析 糖尿病 CLIA 

分 类 号：R587.104[医药卫生—内分泌]