复方延胡索颗粒的制备、质控与初步戒毒作用的研究  

Pharmaceutics and quality control of Compound Corydalis Granule and preliminary study of its effects on morphine-dependent rats

作　　者：居红卫[1] 蒋雪涛[1] 钟延强[1] 李国栋[1] 高申[1] 

机构地区：[1]第二军医大学药学院药剂学教研室,上海200433

出　　处：《第二军医大学学报》2001年第3期290-292,共3页Academic Journal of Second Military Medical University

摘　　要：目的 :研究复方延胡索颗粒的制备、质量控制方法 ,观察该制剂对吗啡依赖鼠的治疗作用。方法 :选用黄芪、延胡索、甘草、远志、酸枣仁、石菖蒲、芸香等七种中药组方制备复方延胡索颗粒 ,以有效部位总生物碱及有效成分四氢帕马丁的含量作为制备方法的筛选指标及复方延胡索颗粒制剂的质控指标。总生物碱的含量用非水电位滴定法测定 ,四氢帕马丁的含量以单波长反射锯齿式薄层扫描外标两点法测定。以该中药制剂治疗吗啡依赖性大鼠自然戒断模型体质量变化百分率作为初步戒毒作用的观察指标。 结果 :四氢帕马丁在 1～ 6μg范围内线性关系良好 (r=0 .9992 ) ,其仪器精密度、同板精密度良好(RSD<3.0 % ) ,且在显色后 0 .5～ 6 h内结果稳定 ,制剂的加样回收率约为 97%。复方延胡索颗粒中剂量组 (0 .6 g/ kg)和高剂量组 (1.2 g/ kg)对吗啡依赖鼠的体质量下降可产生显著治疗作用 (P<0 .0 5 ,P<0 .0 1)。结论 :本制剂一定剂量可有效控制吗啡身体依赖性大鼠戒断后的体质量下降 ,对吸毒者可能有促进康复的作用。Objective:To research proper pharmaceutics method for Compound Corydalis Granule (CCG), and study the effect of CCG on treating morphine withdrawal syndrome. Methods:The prescription of CCG included Astragalus, Corydalis, Glycyrrhiza, Ziziphus, Polygala, Acrous, Cymbopogon and so on. Total alkoloids in CCG were measured by nonaqueous potential titrations and tetrahydropalmatine by thin layer chromatography(TLC). Detoxification on the morphine dependent rats was evaluated by its body mass change. Results:TLC's linear bearing was good( r =0.999 2)and its accuracy of instrument and same layer were also good(RSD<3.0%). The point's area was stable from 0.5 to 6 h after being developed visible. Middle dose group (0.6 g/kg) and high dose group(1.2 g/kg) of CCG could decrease the body mass lose significantly ( P <0.05, P <0.01). Conclusion: Certain dose of CCG is effective on treating morphine withdrawal syndrome in rats. [

关 键 词：四氢帕马丁 生物碱类 非水电位滴定法 薄层扫描法 吗啡依赖 鼠 戒毒 中医药治疗 复方延胡索颗粒 

分 类 号：R259.955[医药卫生—中西医结合]