柠檬酸铵对紫杉醇生物合成途径的诱导作用研究  

Elicitation of taxol biosynthetic pathway by ammonium citrate

作　　者：未作君[1] 苗志奇[1] 元英进[1] 

机构地区：[1]天津大学化工学院制药工程系,天津300072

出　　处：《中草药》2001年第3期213-215,共3页Chinese Traditional and Herbal Drugs

基　　金：国家教委"跨世纪优秀人才培养计划"基金;天津市科委项目资助

摘　　要：目的　确定柠檬酸铵在紫杉醇生物代谢途径的作用位点。方法　分别在 0 ,0 .1,1,10 ,2 0μmol/L的柠檬酸铵诱导下 ,观察紫杉烷类物质产量的变化 ,并通过生物代谢途径进行定性分析。结果　紫杉醇的产量提高近 3倍 ,而 10 - DAB(10 - deactyl baccatin )与 baccatin 的浓度下降。结论　柠檬酸铵提高了紫杉醇合成途径中从 bac-catin 与紫杉醇间的反应速度 ,即其作用位点位于 baccatinObject To study the eliciting effect of ammonium citrate (AC) on the yield of taxol Methods The study was carried out on plant cell cultures with different concentrations of added AC Results Yield of taxol was increased by 3 times under 10 μmol/L AC elicitation, while the concentrations of 10 deacetyl baccatin Ⅲ and baccatin Ⅲ were simultaneously decreased Conclusion By analyzing the changing characters between substrate and enzyme in the simplified taxol synthetic pathway AC was considered to play the role in increasing the reaction rate from baccatin Ⅲ to taxol

关 键 词：紫杉醇 柠檬酸铵 作用位点 植物细胞培养 生物合成 中药 

分 类 号：R284.3[医药卫生—中药学] TQ461[医药卫生—中医学]