槐胺碱对皮肤移植大鼠T淋巴细胞的影响  

Effect of sophoramine on T lymphocytes in rats of skin allografting

作　　者：张佩[1] 纪丽丽 王晨光[1] 李淑华[1] 

机构地区：[1]锦州医学院免疫微生物学教研室,辽宁锦州121001 [2]锦州市妇婴医院病理科,辽宁锦州121004

出　　处：《中草药》2001年第3期230-232,共3页Chinese Traditional and Herbal Drugs

基　　金：辽宁省教委资助

摘　　要：目的　研究槐胺碱 (SA)对移植排斥反应的影响并探讨其发生机制。方法　以同种皮肤移植大鼠作为动物模型 ,免疫荧光浊测定 T淋巴细胞及其亚群数量 ;MTT比色法测定 T淋巴细胞增殖反应。结果　 SA减少 CD3、CD4细胞数 ,降低 CD4/ CD8,抑制 T淋巴细胞的增殖 ,延长移植物存活时间。结论　 SA对移植排斥反应有抑制作用 ,此作用与其减少 T淋巴细胞数量并抑制Object In order to obtain further evidence of therapeutic efficacy of sophoramine (SA) *, its effect on transplantation rejection was studied with simultaneous exploration of its mechanism of action Methods Number of T lymphocyte and its sub sets of skin allografted rat model were determined by immunofluorescence technique, and proliferation of T lymphocyte was assessed by thiazolyl blue (MTT) assay Results SA reduced the CD 3 and C 4 cell counts, lowered CD 4/C 8 ratio, inhibited T lymphocyte proliferation and prolonged the survival time of skin allografted rat Conclusion SA could inhibit transplantation rejection by inhibiting the function of T lymphocyte with simultaneous reduction of lymphocyte count

关 键 词：槐胺碱 皮肤移植 T淋巴细胞 大鼠 移植排斥反应 

分 类 号：R622[医药卫生—整形外科] R285.5[医药卫生—外科学]