紫外线对Bt伴孢晶体的损伤和腐植酸的保护作用  

THE INACTIVATION OF BACILLUS THURINGIENSIS PARASPORAL CRYSTALS BY ULTRAVIOLET AND THE PROTECTIVE ACTION OF HUMIC ACIDS

作　　者：王文军[1] 钱传范[1] 申继忠[1] 程雪梅 

机构地区：[1]中国农业大学应用化学系

出　　处：《植物保护学报》2001年第1期49-54,共6页Journal of Plant Protection

摘　　要：用SDS-PAGE电泳分析和生物测定等方法研究了紫外线对苏云金芽孢杆菌(Bacillus thuringiensis,简称Bt)伴孢晶体的损伤及腐植酸对Bt伴孢晶体的保护作用。结果表明,紫外线对Bt伴孢晶体有明显的损伤,伴孢晶体经紫外线辐射3h后溶解性能及生物活性基本丧失。腐植酸对紫外线有较强的吸收作用,能有效地减轻紫外线对Bt伴孢晶体的损伤。The ultraviolet-mediated impairment and inactivation of purified HD-1 parasporal crystals were studied by the means of SDS-PAGE and bioassay. HD-1 parasporal crystals which were irradiated by ultraviolet light for less than 3h lost the normal ability of dissolution in aqueous base and in proteases from the midgut of Helicaverpa armigera ( Hubner) , and lost the original insecticidal activity against H. armigera. Humic acids, soluble natural substances, characterized by the strong absorption of the light of the 200 - 400nm range, and acted as the protectants of parasporal crystals, were very effective on retarding the impairment and inactivation of Bt crystals by ultraviolet. HD-1 crystals with 0.1% humic acids as ultraviolet absorbers exhibited the good solubility in aqueous base and proteases and retained 79.7% of their original insecticide activity after irradiated by ultraviolet light for 6h.

关 键 词：苏云金芽孢杆菌 伴孢晶体 紫外线 腐植酸 生物活性 保护作用 

分 类 号：S482.39[农业科学—农药学] S476.11[农业科学—植物保护]