抗HSA与微晶纤维素的固定化  

Immobilization for Anti-Human Serum Albumin and Cellulose Particles

作　　者：何俊[1] 李勇[1] 但德忠[2] 张宏[1] 乔小蓉[1] 王方强[2] 

机构地区：[1]华西医科大学 [2]四川大学环境科学与工程学院,成都610041

出　　处：《现代临床医学生物工程学杂志》2001年第1期16-17,共2页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然科学基金资助课题! (39570 2 1 8)

摘　　要：采用溴化氰化学修饰法制备抗HSA -微晶纤维素固相抗体 .对制备条件进行了优化 ,以修饰温度 2 0℃、抗体浓度 3.5mg/mL为固定化最佳条件 ;并对固相抗体进行了扫描电镜测定。Anti-human serum albumin(Anti-HSA) was immobilized on microcrystalline cellulose activated by CNBr. The experimental conditions were optimized. The optimum temperature and concentration of the antibodies for immobilization were 20℃ and 3.5mg/mL, respectively. The immobilized antibody were determined by SEM and applied in fluoroimmunoassay.

关 键 词：兔 抗HSA 微晶纤维素 固定化 荧光免疫分析 固相抗体 

分 类 号：R318.08[医药卫生—生物医学工程]