大豆对SMV3号株系的抗性遗传分析及抗病基因的RAPD标记研究  

Inheritance of Resistance to SMV3 and Identification of RAPD Marker Linked to the Resistance Gene in Soybean

作　　者：郑翠明[1,2] 常汝镇[1,2] 邱丽娟[1,2] 

机构地区：[1]中国农业科学院作物品种资源研究所 [2]农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《中国农业科学》2001年第1期14-18,共5页Scientia Agricultura Sinica

基　　金：国家"九五"重点科技攻关项目!( 96-C0 1-0 1-0 6);国家"九七三"项目!( G19980 10 2 0 3 )

摘　　要：利用高抗东北 SMV3号株系的大豆品系 95 - 5 383与 4个感病品种 (系 ) HB1、铁丰 2 1、Am soy、William s和抗病品种 PI486 35 5配制 5个杂交组合 ,对各组合的 F1 、F2 代接种 SMV鉴定抗性。结果表明 ,95 - 5 383与各感病品种杂交组合的 F1 代表现为感病 ,F2 群体分离比例为 3感 (花叶 +顶枯 )∶ 1抗 ,表明 95 - 5 383对 SMV3号株系的抗性受一对隐性基因控制。 95 - 5 383× PI486 35 5的 F2 代接种后有感病植株分离 ,表明二者对 SMV3的抗性基因不等位。利用BSA法对 95 - 5 383× HB1的 F2 代进行鉴定 ,筛选出 RAPD引物 OPN11在 95 - 5 383和抗池扩增出 OPN11980 片段 ,在HB1和感池扩增出 OPN111 0 70 片段 ,在 F1 同时扩增出 OPN11980 和 OPN111 0 70 。用该引物分析 95 - 5 383× HB1的 F2 个体 ,共显性的 RAPD标记 OPN11980 /1 0 70 与 95 - 5 383抗病基因的遗传距离为 2 .1c M。One SMV resistant soybean line(95 5383) was crossed with four susceptible soybean varieties/line(HB1, Tiefeng21, Amsoy, Williams) and one resistant variety PI486355. Their F 1 and F 2 individuals were identified for SMV resistance by inoculation with SMV3. The results showed that in the four crosses of resistant×susceptible, F 1 were susceptible and the ratio of F 2 populations was 1 resistant∶3 susceptible (mosaic and necrosis), indicating that 95 5383 carries one recessive gene that confer resistance to SMV. There is a segregation of susceptibility in F 2 progenies from the cross of 95 5383×PI486355, indicating that the SMV resistance genes in 95 5383 and PI486355 are located at different loci. By bulked segregant analysis(BSA) in F 2 populations of 95 5383×HB1, one codominant RAPD marker OPN11 980 /1070 closely linked to SMV resistance gene amplified with RAPD primer OPN11 was identified. DNA fragment OPN11 980 was amplified in resistant parent 95 5383 and resistant bulk, OPN11 1070 was amplified in susceptible parent HB1 and susceptible bulk. OPN11 980/1070 was amplified in F 1. Identification of the markers in F 2 plants showed that the codominant marker OPN11 980/1070 is closely linked to the SMV resistance gene in 95 5383, with genetic distance of 2.1cM.

关 键 词：大豆 大豆花叶病毒 抗性遗传 RAPD标记 抗病基因 

分 类 号：S565.1[农业科学—作物学]