乙型肝炎肝组织Fas,FasL和HBV抗原的表达  

Study on expression of Fas/FasL and HBV antigens in liver tissue of patients with hepatitis B

作　　者：王慧芬[1] 胡瑾华[1] 朱克顺[1] 张玲霞[1] 王松山[1] 王林杰[1] 

机构地区：[1]北京解放军302医院,北京100039

出　　处：《中华实验和临床病毒学杂志》2001年第1期12-15,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　评价Fas和FasL介导细胞凋亡在乙型肝炎肝损伤中的作用及其与HBV抗原的关系。方法　应用免疫组化方法检测了 6 2例乙型肝炎患者肝组织内的Fas、FasL和HBsAg、HBcAg ,并以 6例正常肝组织为对照。结果　Fas/FasL在正常肝组织内无表达 ;Fas主要在乙型肝炎患者肝细胞质内表达 ,5 8例阳性 (93 5 % ) ;FasL在肝内浸润的单个核细胞和肝细胞质内均可见到 ,37例阳性 (5 9 7% )。结论　Fas/FasL的表达程度与肝组织活动性炎症程度密切相关 ;Fas/FasL介导的细胞凋亡可能在乙型肝炎时肝损伤中起重要作用 ;Fas/FasL表达程度与肝内HBV抗原HBsAg。Objective To evaluate the role of apoptosis mediated by Fas/FasL in the liver of hepatitis B and the expression status of Fas,FasL,and HBV antigens.Methods We studied the expression of Fas antigen,Fas\|ligand (FasL) and hepatitis B virus(HBV) antigens(HBsAg and HBcAg)in the livers of 62 patients with hepatitis B using immunohistochemistry ABC method.Six normal liver samples were used as control.Results Hepatocytes in normal liver had no Fas/FasL expression,but in the cases of hepatitis B,Fas was expressed mainly in the cytoplasm in 58 cases(93\^5%);FasL was observed in infiltrating mononuclear cells and in the cytoplasm of hepatocytes in 37 cases (59\^7%).The expressions of Fas/FasL were closely related to the liver histological inflammation degrees.Conclusion The apoptosis mediated by Fas/FasL may play an important role in the hepatocellular injury in hepatitis B.No correlation was found between the degrees of Fas/FasL expression and the presence of HBsAg and HBcAg in the liver.

关 键 词：FAS抗原 FAS配体 乙型肝炎 发病机理 病理学 

分 类 号：R512.6[医药卫生—内科学]