广州地区肝炎患者血清中庚型肝炎病毒核酸检测和病毒核苷酸测序  

Detection and partial nucleotide sequence analysis of hepatitis G virus in sera of patients with hepatitis in Guangzhou

作　　者：唐漾波[1] 163.net 李涤蓉[1] 刘国莲[1] 石裕明[1] 魏绍静[1] 冼超[1] 吴婉芬[1] 

机构地区：[1]广州市第八人民医院传染病研究所肝炎室

出　　处：《中华实验和临床病毒学杂志》2001年第1期24-26,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：广州市医药卫生青年科研计划资助!(96 -16 )

摘　　要：目的　了解广州地区肝炎患者中庚型肝炎病毒 (HGV)的感染情况及基因型特点。方法　采用逆转录 套式聚合酶链反应 (RT nestedPCR)方法检测肝炎患者血清中HGVRNA ,引物位于HGV基因组的非编码区 ,并对扩增产物进行直接测序。结果　 2 5 1例急慢性肝炎血清中共检出 2 5例HGVRNA阳性 ,总阳性率为 9 96 % ,其中 5 6例非甲～戊型肝炎中检出 4例阳性 (7 14% ) ,77例乙型肝炎 (HB)中检出 8例阳性 (10 39% ) ,118例丙型肝炎 (HC)中检出 13例阳性 (11 17% ) ;2株HGV广州株核苷酸之间的同源性为 98 0 % ,与非洲株的同源性分别为 81 7%和 84 2 % ,与美国株的同源性均为 91 1%。结论　广州地区肝炎患者中存在HGV感染 ,2株HGV广州株可能为同一基因型 。Objective To investigate hepatitis G virus (HGV) infection in hepatitis patients and the gene type of HGV in Guangzhou.Methods Reverse transcriptase\|nested polymerase chain reaction(RT\|nested PCR) method using primers locating in noncoding region of HGV genome was used for detecting HGV RNA in sera of hepatitis patients and the amplified products were directly sequenced.Results 25 cases were HGV RNA positive in 251 sera of acute and chronic hepatitis(9\^96%),of which 4 cases were positive in 56 sera with hepatitis non A\|E(7\^14%),8 cases were positive in 77 sera with hepatitis B(10\^39%),13 cases were positive in 118 sera with hepatitis C(11\^17%).The identity of nucleotide sequence was 98\^0% between 2 Guangzhou HGV isolates,both were 91\^1% with American isolate,81\^7% and 84\^2% respectively between 2 Guangzhou isolates and African isolate.Conclusion HGV infection exists in hepatitis patients,2 Guangzhou HGV isolates may belong to the same gene type and both of them have a high homology with American HGV isolate.

关 键 词：庚型肝炎病毒 聚合酶链反应 碱基序列 乙型肝炎 丙型肝炎 

分 类 号：R446.5[医药卫生—诊断学]