小鼠对表达狂犬病毒3aG株糖蛋白的重组复制缺陷型腺病毒的免疫应答  

Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain

作　　者：李文辉[1] 张云[1] 王树蕙[1] 刘力[1] 

机构地区：[1]北京中国医学科学院中国协和医科大学基础医学研究所,病原室100005

出　　处：《中华实验和临床病毒学杂志》2001年第1期61-65,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"8 6 3"计划资助!(86 3 10 1 0 5 0 4 0 3)

摘　　要：目的　研究表达狂犬病毒 3aG株糖蛋白 (GP)的重组复制缺陷型腺病毒Ad/GP′及Ad/GP免疫小鼠后所产生的特异性体液免疫应答 ,体外脾细胞增殖反应 ,及免疫小鼠对狂犬病毒致死性颅内攻击的保护力。方法　 1× 10 7pfu重组病毒经腹腔对小鼠进行基础和加强免疫 ,以快速荧光灶抑制实验 (RFFIT)方法测定小鼠血清狂犬病毒特异性中和抗体滴度 ,[3H]TdR掺入DNA法测定体外脾细胞增殖反应 ;小鼠致死性CVS狂犬病毒颅内攻击测定重组病毒免疫对小鼠的保护效果。结果　RFFIT方法测定免疫小鼠血清中和抗体滴度分别为Ad/GP :0 75IU/ml,Ad/GP’ :2 6IU/ml;[3H]TdR掺入法测定重组病毒免疫组小鼠脾细胞体外受到特异GP抗原刺激后 ,增殖增强 2倍以上 ;86 7%的Ad/GP′免疫小鼠及 6 6 7%的Ad/GP免疫小鼠可抵抗约 30LD50 CVS狂犬病毒的颅内攻击。Objective To evaluate the immune response of mice immunized with E1,E3 deleted replicative deficient recombinant adenovirus which can express glycoprotein of Chinese 3aG rabies virus. Methods Rapid fluorescent focus inhibition test (RFFIT) was used to determine the titer of neutralizing antibodies; specific antigen induced spleen lymphocyte proliferation was determined by in vitro \+3H\|TdR incorporation assay, and lethal intracerebral challenge with CVS rabies virus was used to evaluate the protective effectiveness of the recombinant virus as candidate vaccine. Results Mice immunized by 1×10 7 recombinant virus developed high levels of rabies virus neutralizing antibodies (VNA). The spleen lymphocyte of the immunized mice also demonstrated remarkable increased proliferation after stimulation with inactivated rabies virus; 70%～90% of the mice immunized by the recombinant virus were protected from lethal intracerebral challenge with CVS rabies virus.Conclusion E1, E3 deleted replication defective recombinant adenovirus containing 3aG glycoprotein gene may be developed as effective rabies vaccine to control rabies in China.

关 键 词：重组腺病毒 狂犬病毒 免疫应答 

分 类 号：R392.3[医药卫生—免疫学]