柯萨奇B组病毒IgM抗体特性研究  

Study on specificity of IgM antibody response in patients with Coxsackie virus B infection

作　　者：张霆[1] 马官福[1] 马连华[1] 刘哲伟[1] 崔小岱[1] 许诤[1] 

机构地区：[1]首都儿科研究所北京市感染与免疫中心实验室,北京100020

出　　处：《中华实验和临床病毒学杂志》2001年第1期66-68,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究柯萨奇B组病毒 (CVB)感染后患者急性期CVB IgM抗体的反应特性。 方法　对临床确诊为CVB感染的患者急性期血清用免疫印迹法检测CVB IgM抗体。结果　患者的急性期血清IgM抗体均仅针对CVB的VP1抗原反应。经ELISA与免疫印迹方法的比较表明 :用免疫印迹法检测的CVB IgM抗体可对CVB各型病毒抗原反应 ,具有CVB各型病毒之间的交叉反应性。而同时 ,在 16例ELISA检测反应阴性的健康人血清中未见有CVB的VP1特异性的IgM抗体存在。结论　临床感染CVB的患者急性期 ,其血清中的特异性的IgM抗体是针对CVB的VP1抗原 ,而且 。Objective To study the specificity of IgM antibody response in patients with Coxsackie virus B infection. Methods A Coxsackie virus B specific immunoblot assay was established for detection of CVB IgM antibody response in patients with recent CVB infection. Results Of 28 patients' serum samples, positive by ELISA captured CVB IgM test, analysis results of specific IgM response defined by immunoblot assay revealed VP1 protein specific which was crossly reactive among CVB group. Conclusion This finding suggested that VP1 protein in Coxsackie virus B group potentially functioned as a predominant antigen inducing detectable IgM antibody following CVB infection.

关 键 词：柯萨奇病毒B组 免疫印迹法 IGM 

分 类 号：R392.12[医药卫生—免疫学]