不育男性、少精子症病人精子肌酸激酶活性及其同工酶组成的研究  

Activity of creatine kinase and the relative content of M-CK isoform in spermatozoa from Infertile men

作　　者：罗比[1] 王萍[1] 李晓梅[1] 

机构地区：[1]四川省计划生育科研所生化室,成都610041

出　　处：《中国男科学杂志》2001年第1期33-36,共4页Chinese Journal of Andrology

摘　　要：采用酶动力学分光光度法和琼脂糖电泳凝胶光密度扫描分别对正常生育男性 (2 7例 )、少精子症患者(14例 )和精子计数大于 2× 10 7/ml的不育男性 (80例 )的精子肌酸激酶 (CK)总活性和M CK及B CK同工酶的相对含量进行了测定。结果CK总活性分别为 116± 5 6 .5mU/ 10 8精子 ( x± 2SE) ,5 40± 36 7mU/ 10 8精子和 16 8±5 1.7mU/ 10 8精子。与正常人比较 ,少精子症组显著升高 (P <0 .0 5 ) ,不育组略有升高 ,但与正常组之间的差异无显著性。正常对照组精子M CK和B CK含量分别占总活性的 30 .5± 4.35 %和 37.5± 6 .14% ( x±ZSE) ,少精子症组和不育男性组的M CK含量均明显降低 ,分别是 18.0± 2 .36 %和 19.0± 1.32 % 。Total activity of creatine kinase(CK) and the relative contents of M?CK and B?CK isoenzyme in spermatozoa from oligozoospermic patients( n =14),infertile men( n =80)and controls( n =27)have been determined by using kinetic spectrophotometry and density scanning on agarose gel after electrophoretic separation,respectively.The total activity of CK in spermatozoa were 116±56.5 mU/10 8 sp(mean±2SE),540±367 mU/10 8 sp and 168±51.7 mU/10 8 sp in control,oligo and infertile groups,respectively.The CK activity in oligozoospermic patients increased significantly compared with that of controls( P <0.05).No significant difference in sperm CK between infertile and control groups has been found.The results of CK isoenzyme analysis showed that the relative contents of M?CK and B?CK are 30.5±4.35% and 37.5± 6.14 %(mean±2SE) in normal controls,while the M?CK contents in infertile men and oligozoospermic patients were much lower(19.0±1.32% and 18.9±2.45%,respectively),implying a close correlation between this differentiation marker in cytoplasma and the sperm maturation and hence,the potential of sperm insemination.

关 键 词：肌酸(磷酸)激酶 同工酶 精子 少精子症 男性不育症 

分 类 号：R698.2[医药卫生—泌尿科学]