中耳细菌感染诱导的急性期HSP-70相关反应的免疫组织化学研究  

Immunocytochemical research of HSP-70 correlative response in acute stage induced by bacterial infected middle ear

作　　者：周永清[1] 邹静[1] 毕爱芳[1] 尚耀东[1] 

机构地区：[1]石家庄白求恩国际和平医院全军耳鼻咽喉病中心,050082

出　　处：《耳鼻咽喉（头颈外科）》2001年第1期42-47,共6页Chinese Arch Otolaryngology-Head Neck Surg

摘　　要：探讨中耳细菌感染急性期时 ,哺乳动物中、内耳热休克反应的部位 ,以及中耳细菌感染诱生的热休克蛋白是否可能引发内耳自身免疫损伤。运用中耳注射肺炎克雷伯杆菌制成豚鼠中耳急性感染动物模型 ,分别于接种后第 1、3、5、7天处死动物取材。应用免疫组化技术 ,研究了中耳粘膜和耳蜗表达热休克蛋白 70 (HSP- 70 )的部位。结果表明 :正常状态下 ,中耳粘膜表层的上皮细胞和内耳膜迷路血管纹、螺旋韧带、Corti氏器均有弱的阳性反应 ,感染应激后 ,上述同样部位均有强的阳性显色。不同取材时段显示的阳性位置无差异。说明在中耳急性细菌感染期 ,中耳粘膜和内耳组织均表达了同源 HSP- 70蛋白分子 ,这些同源 HSP- 70为引发内耳自身免疫损伤提供了物质基础。To illustrate the location of HSP 70 (heat\|shock protein\|70)correlative response in acute stage between middle and inner ear when mammalian middle ear infected by bacteria, and investigate the possibility to cause autoimmune inner ear injure by HSP 70 that induced by otitis media. An animal model of acute otitis media was established by inoculating Klebsiella pneumoniae (K.p) into middle ear of guinea pigs. Animals were sacrificed 1,3,5 and 7days after inoculation.Investigation of the locations of HSP 70 in middle ear mucosa and cochlea structures were detected by immunocytochemical technique .The results showed that light stain of HSP 70 existed in the superficial epithelium cells of normal mucosa and cochlea structures such as stria vascularis, spiral ligament and Corti′s organ.Heavy stain appeared in the same sites in the infected ears . No different positive sites was observed in all animals.Our findings suggested that HSP 70 were expressed in middle ear mucosa and inner ear structures during acute middle ear infective stage.The HSP 70 provide a material base for causing autoimmune inner ear injure during middle ear bacteria infection.

关 键 词：中耳 细菌感染 热休克蛋白质类 免疫组织化学 

分 类 号：R764.2[医药卫生—耳鼻咽喉科]