寻常性银屑病患者外周血单一核细胞产生IL-2及IL-4能力的研究  

Investigation of Production of Interleukin-2 and Interleukin-4 by Peripheral Blood Mononuclear Cells in Patients with Psoriasis Vulgaris

作　　者：杨竹生[1] 李玛琳[2] 何黎[1] 王朝凤[1] 吕昭萍[1] 

机构地区：[1]昆明医学院第一附属医院皮肤科,云南昆明650032 [2]昆明医学院天然药物研究重点实验室

出　　处：《中国皮肤性病学杂志》2001年第2期76-78,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的 探讨寻常性银屑病患者外周血单一核细胞（ＰＢＭＣｓ）产生白介素２（ＩＬ－２）及白介素４（ＩＬ－４）的能力与病程演变的关系。方法 用生物活性法检测产生ＩＬ－２、双抗体夹心ＥＬＩＳＡ法检测产生ＩＬ－４的水平。结果与１２例健康人比较，患者组ＩＬ－２低于对照组（Ｐ＜０．０１），静止期组较进行期组降低更明显；患者组ＩＬ－４低于对照组（Ｐ＜０．０５），进行期组明显低于对照组（Ｐ＜０．０１），而静止期组与对照组比较差异无显著性（Ｐ＞０．０５）。结论患者从进行期向静止期演变可能与ＰＢＭＣｓ产生ＩＬ－２及ＩＬ－４的能力改变有关。Objective To study the correlation between the clinical evolution and the mitogen-stimulated production of in- terleukin-2(IL-2) and interleukin-4(IL-4) in vitro of peripheral blood mononuclear cells(PBMCs) in patients with psoriasis vul- garis. Methods The production of IL-2 was analyzed with bicactivity assay. The production of IL-4 was assayed by enzyme- linked immunosorbent assay(ELISA) test. For comparison, PBMCs from 12 healthy controls were tested equally. Results The IL-2 production of patients with psoriasis was more decreased than these of the healthy contrls(P < 0. 01 ), especially in patients with static psoriasis. The production of IL-4 was inhibited in patients with active psoriasis(P< 0. 01 ), while it became normal in patients with static psoriasis(P >0. 05). Conclusion There is a possible correlation between the clinical evolution of psoriasis and the production of IL-2 and IL-4 of PBMCs.

关 键 词：寻常性银屑病 单一核细胞 外周血 白介素2 白介素4 

分 类 号：R758.630.3[医药卫生—皮肤病学与性病学]