系统性红斑狼疮外周血单一核细胞Fas和FasL mRNA的表达  

Expression of Fas and FasL mRNA in the Peripheral Blood Mononuclear Cells of the Patients with Systemic Lupus Erythemato- sus

作　　者：弓娟琴[1] 陈志强[1] 胡兹嘉[1] 李文忠[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所,江苏南京210042

出　　处：《中国皮肤性病学杂志》2001年第2期79-81,共3页The Chinese Journal of Dermatovenereology

基　　金：中国医学科学院皮肤病研究所(所)院基金资助课题

摘　　要：目的 探讨系统性红斑狼疮（ＳＬ）患者外周血淋巴细胞Ｆａｓ及其相应配体ＦａｓＬ的表达水平及在ＳＬＥ发病中的作用。方法　应用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测３４例活动期ＳＬＥ患者和３０例正常人外周血单一核细胞（ＰＢＭＣ）中 Ｆａｓ和 ＦａｓＬ ｍＲＮＡ的表达水平。结果活动期 ＳＬＥ患者 ＰＢＭＣ中 ＦａｓＬ的阳性检出率为 ９１． １８％，明显高于正常对照组（６３．３０％），差异非常显著（Ｐ＜０．０１）；Ｆａｓ的阳性检出率与对照组相比无明显差异（Ｐ＞０．０５）。活动期ＳＬＥ患者．ＰＢＭＣ中Ｆａｓ和ＦａｓＬ ｍＲＮＡ的平均表达水平均高于正常对照组，差异显著（Ｐ＜０．０５）。结论Ｆａｓ和ＦａｓＬ的高水平表达可能与ＳＬＥ患者外周血Ｔ淋巴细胞活化、凋亡增加有关，Ｆａｓ介导的淋巴细胞凋亡可能参与了ＳＬＥ的发病过程。Objective To investigate the levels of Fas and Fas ligand mRNA expression in the patients with systemic lupus erythematosus. Methods Applying reverse transcription-polymerase reaction(RT-PCR) technique to analyze Fas and FasL mR- NA expression in the peripheral blood mononuclear cells(PBMC) from 34 active SLE patients and 30 cases of healthy subjects. Results The SLE patients with positive rate of FasL expression accounted for 91. 18% of the SLE patients in active stage,com- pared with that of normal controls(63. 30% ),The difference was markedly markedly significant(p< 0. 01). The mean levels of Fas and FasL mRNA expression in active SLE group were increased(p< 0. 05), there was no statistical significance in the positive rate of Fas expression between patients with active SLE and normal controls(P>0. 05). Conclusions The higher expression of Fas and FasL may contribute to the activation of lymphocytes and/or the increased apoptosis of lymphocytes in patients with systemic lu- pus erythematosus. Fas-mediated apoptosis may be involved in the pathogenesis. of SLE.

关 键 词：系统性红班狼疮 单一核细胞 外周血Fas FASL 

分 类 号：R593.241[医药卫生—内科学]