反义c-jun表达质粒对血管平滑肌细胞表型转化标志的影响  

Effects of c-jun Antisense Gene Expression Plasmids on Phenotypic Switch Markers of Rat Vascular Smooth Muscle Cells

作　　者：李琦[1] 温进坤[1] 韩梅[1] 

机构地区：[1]河北医科大学基础医学研究所生物化学研究室,河北省石家庄市050017

出　　处：《中国动脉硬化杂志》2001年第1期6-9,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金!( 3 9970 2 74);河北省自然科学基金!( 3 0 13 5 8)资助项目

摘　　要：为研究c jun反义RNA对血管平滑肌细胞表型转化的影响 ,本文应用可表达c jun反义RNA的真核细胞表达载体转染大鼠血管平滑肌细胞 ,采用Northern印迹、Western印迹和氚标胸腺嘧啶核苷掺入实验 ,观察反义c jun对血管平滑肌细胞表型标志基因平滑肌α 肌动蛋白、平滑肌胚胎型肌球蛋白重链和骨桥蛋白表达和DNA合成的影响。结果发现 ,在血管平滑肌细胞中表达的c jun反义RNA可使血管平滑肌细胞去分化标志基因平滑肌胚胎型肌球蛋白重链和骨桥蛋白mRNA表达较对照分别下降 5 0 %和 95 % ,骨桥蛋白水平较对照下降 75 % ,同时血管平滑肌细胞的DNA合成受到显著抑制 ,实验组氚标胸腺嘧啶核苷掺入值较对照降低 37%。c jun反义RNA对血管平滑肌细胞分化标志基因平滑肌α 肌动蛋白的表达无明显影响。提示c jun基因表达产物在维持血管平滑肌细胞于去分化状态中起重要作用。Aim To study the effects of c jun antisense gene on phenotypic switch of vascular smooth muscle cells (VSMC). Methods Eukaryotic expression vectors which could transcript into c jun antisense RNA were transfected into rat VSMC, Northern blotting, Western blotting and 3 H TdR incorporation were used to observe the effects of c jun antisense RNA on the expression of phenotypic marker genes SM α actin, SMemb, osteopontin and DNA synthesis of VSMC. Results By the c jun antisense RNA expressed in VSMC, compared with control group, the mRNA expression of dedifferentiated marker genes SMemb and osteopontin decreased 50% and 95% respectively, the osteopontin decreased 75%, and the DNA synthesis of VSMC was evidently inhibited, the 3 H TdR incorporation decreased 37%, but the effect of c jun antisense RNA on differentiated marker gene SM α actin had not been observed. Conclusion The expressive products of c jun gene was important for VSMC to keep dedifferentiated.

关 键 词：jun基因 反义核苷酸 表型 血管平滑肌 遗传标志 骨桥蛋白 基因表达 

分 类 号：Q527[生物学—生物化学]