球囊损伤兔主动脉后核因子-κB活性的动态变化  

Dynamic Changes of NF-κB Activity in Balloon-Injured Aortic Arteries of Rabbits

作　　者：李建军[1] 李庚山[1] 黄从新[1] 江洪[1] 许家俐[1] 唐其柱[1] 夏豪[1] 王晶[1] 

机构地区：[1]武汉大学亚太医院暨湖北省人民医院心内科,湖北省武汉市430060

出　　处：《中国动脉硬化杂志》2001年第1期34-36,共3页Chinese Journal of Arteriosclerosis

摘　　要：为探讨经皮球囊损伤兔主动脉内膜后血管组织中核因子 -κB活性的动态变化 ,选日本大白兔 18只 ,经股动脉插入 4FForgarty导管至主动脉 ,充盈球囊 ,回拖球囊至髂总动脉 ,反复 3次 ,复制球囊损伤主动脉内膜的动物模型。于损伤后 0、12、2 4、48和 72h处死动物 ,提取腹主动脉壁组织胞核蛋白 ,电泳流动漂移技术检测核因子 -κB活性的动态变化。结果表明 ,正常主动脉组织中无明显的核因子 -κB激活现象 ,球囊损伤主动脉内膜后即刻在血管组织有一定水平的核因子 -κB激活 ,12h达高峰 ,持续 48h ,72h逐渐恢复到对照水平。密度扫描结果提示 ,核因子 -κB活性于球囊损伤内膜后 12h时较对照 (内膜损伤后即刻 )增高 3.7倍 ,2 4h增加 2 .5倍 ,48h增加 1.4倍 ,72h逐渐恢复到对照水平。提示球囊损伤兔主动脉内膜后血管组织中核因子 -κB活性异常激活 ,可能是血管平滑肌细胞增殖的启动机制之一。Aim To investigate the dynamic changes of nuclear factor-κB(NF-κB)activity in balloon-injured arteries,rabbit aortas(n=18)were subjected to abrasion injury using 4F Forgarty catheter. The arterial tissues were collected and nuclear protein of the tissue was extracted at 0, 12, 24, 48 and 72 h following injury. NF-κB activity in balloon-injured arteries were examined by electrophoretic mobility shift assay. No detectable NF-κB activity was found in normal arteries of rabbits,while NF-κB binding activity in balloon-injured arteries increased apparently,which was detected immediately following injury,peaked at 12 hours,lasted 48 h, returned to baseline at 72 h. The results of density scan for binding blot showed that NF-κB activity was enhanced more than 3.7 fold at 12 h(n=4, P<0.01),2.5 fold at 24 h (n=4, P<0.01),and 1.4 fold at 48 h (n=4, P<0.05), compared with baseline activity (0 h)of NF-κB. The present data showed that balloon injury could trigger NF-κB activation in arteries, which was likely to be involved in the proliferation of arterial smooth muscle cells.

关 键 词：核因子-ΚB 球囊损伤 血管平滑肌 

分 类 号：R543[医药卫生—心血管疾病]