胎儿骨髓基质细胞协同外源性细胞因子对脐带血CD34^+细胞的体外扩增作用  

Fetal Bone Marrow Stromal Cells in Cooperation with Exogenous Cytokines Support In Vitro Expansion of Cord Blood CD34^+ Cells

作　　者：韩军[1] 王健民[1] 徐明娟[2] 张明徽[3] 周虹[1] 闵碧荷[1] 

机构地区：[1]第二军医大学长海医院血液科 [2]第二军医大学长海医院妇产科 [3]第二军医大学免疫教研室,上海200433

出　　处：《中国实验血液学杂志》2001年第1期93-94,共2页Journal of Experimental Hematology

基　　金：国家自然科学基金资助 编号 3 980 0 0 5 5

摘　　要：To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.

关 键 词：胎儿 骨髓基质细胞 细胞因子 脐带血 CD34^+细胞 

分 类 号：R331.22[医药卫生—人体生理学]