应用ARDRA技术研究Frankia菌多样性  

STUDY ON GENETIC DIVERSITY OF Frankia BY ARDRA

作　　者：刘忠[1] 吴少慧[1] 周斌[1] 张惠文[1] 张忠泽[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110015

出　　处：《微生物学杂志》2001年第1期20-23,共4页Journal of Microbiology

基　　金：国家自然科学基金资助项目!(39870 0 38);中科院系统与进化特别项目资助

摘　　要：应用原核生物 16SrDNA特异性引物rD1和fD1,对分自 4个分类接种群的 12株纯培养Frankia菌总DNA进行扩增 ,得到 1条长约 15 0 0bp的扩增产物。选用 2种内切酶HinfI ,MspI对扩增产物进行酶切 ,得到稳定的酶切图谱。对图谱的分析结果表明 。S ribosomal DNAs (rDNAs) from 12 Frankia strains belonging to 4 taxonomic inoculation groups were amplified by PCR with primer rD1 and fD1. The fragment of 16s rDNA with about 1 500 bp was obtained. The amplification products were digested by Hinff and MspI respectively. It showed that there are great genetic diversity among Frankia strains by comparing the patterns from the digestion.

关 键 词：FRANKIA 多样性 ARDRA 固氮 放线菌 

分 类 号：Q939.13[生物学—微生物学]