RAPD分析在绢丝昆虫亲缘关系研究中的应用Ⅰ.蓖麻蚕品种间的遗传差异  

Application of RAPD Technique in Genetic Relationship Study of Silk Insect Ⅰ. Genetic Variance in Eri Silkworm

作　　者：左正宏 桂慕燕[2] 王学民[2] 陈元霖[2] 

机构地区：[1]湖南省岳阳卫生学校,岳阳414000 [2]福建省厦门大学生物学系,厦门361005

出　　处：《遗传》2001年第2期128-130,共3页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目!(批准号39870410)

摘　　要：用RAPD技术对蓖麻蚕基因组DNA进行多态性研究，分析了5个蓖麻蚕品种间的遗传差异。结果表明，所采用的40个随机引物中，有27个引物扩增谱带清晰且重复性较好，扩增总片段数达243个，单个引物的扩增片段数在4～17之间，平均为9条，片段大小在033～30kb之间。不同蓖麻蚕品种间的遗传距离(Ｄ)在00683～01603之间，根据Ｄ值，由UPGMA聚类分析软件绘制了它们的聚类分子树。Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among eri sickworm. The genetic variance of five erisickworm was studied. The result showed that: 27 of 40 arbitrary primers could amplify clearly with repeatable bands.243 fragments were obtained.Each primer gave 4～17 bands and the average was 9.The length of the band was 0.33～ 3.0kb. The genetic distance (Ｄ) value between different breeds of Eri Silkworm was 0.0683～0.1603. The D value was used to construct a dendrogram by UPGMA.

关 键 词：蓖麻蚕 RAPD 品种差异 遗传差异 缉丝昆虫 亲缘关系 

分 类 号：S881[农业科学—特种经济动物饲养]