一株能在苜蓿上结瘤的费氏中华根瘤菌  

A-SINORHIZOBIUM FREDLL STRAIN THAT EFFECTIVELY NODULATE MEDICAGO SATIVA

作　　者：张海瑜[1] 张海予[1] 李小红[1] 杨苏声[1] 

机构地区：[1]中国农业大学生物学院微生物系,北京100094

出　　处：《微生物学报》2001年第2期127-132,T001,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金!( 39870 0 4 5);欧盟科研基金项目!(IC1 8CT970 1 91 )资助&&

摘　　要：费氏中华根瘤菌 (Sinorhizobiumfredii) 0 4 2BS分离自新疆的苜蓿根瘤 ,通过交叉结瘤试验 ,发现它既可在苜蓿上又可在大豆上结瘤固氮。 1 6SrDNAPCR RFLP分析表明 ,0 4 2BS与费氏中华根瘤菌模式菌株USDA2 0 5的 4种限制性酶切图谱完全一致。其G +Cmol%为 60 0 ,与费氏中华根瘤菌USDA2 0 5和USDA1 91的DNA同源性分别为 84 9%和89 6% ,表明 0 4 2BS属于费氏中华根瘤菌。应用绿色荧光蛋白基因标记 0 4 2BS ,得到重组菌株 0 4 2BSG。将其接种保定苜蓿和北引 1号大豆 ,并重新分离出根瘤菌 ,利用激光共聚焦荧光显微镜检测到标记基因的表达 ,从而确证了 0 4 2BS能在苜蓿和大豆上结瘤。而且 ,0 4Sinorhizobium fredii\% 042BS was isolated from root nodules of alfalfa (\%Medicago sativa\%) from Xinjiang Region.Nodulation experiments showed that both soybean and alfalfa were nodulated by 042BS effectively.The 16S rDNA PCR\|RFLP analysis was carried out by four restriction endonucleases,and the restriction maps of strain 042BS were identical with those of \%S.fredii\% USDA205.The DNA G+C mol% of strain 042BS was 60 0.The DNA homology between 042BS and \%S.fredii\% USDA205 and USDA191 were 84 9% and 89 6%,respectively.To prove the capability of 042BS to nodulate both soybean and alfalfa,constitutively expressed green fluorescence protein gene(\%gfp\%) was introduced to 042BS,and the recombinant strain 042BSG was obtained.The reisolates from nodules of the soybean and alfalfa inoculated with 042BSG were observed using the confocal laser\|scanning microscope,and the expressions of \%gfp\% were detected,respectively.042BS showed various nodulation capacities with different alfalfa cultivars used.

关 键 词：费氏中华根瘤菌 DNA同源性 绿色荧光蛋白 苜蓿 

分 类 号：Q945.13[生物学—植物学]