新生隐球菌交配型分析试验方法和培养基的研究  

STUDIES ON THE METHODS OF MATING TEST FOR CRYPTOCOCCUS NEOFORMANS'

作　　者：李安生[1] 吕桂霞[1] 沈永年[1] 陈伟[1] 吴绍熙[1] 

机构地区：[1]中国医学科学院皮肤病研究所,南京210042

出　　处：《微生物学报》2001年第2期209-215,T002,共8页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助!( 39670 0 4 4)&&

摘　　要：比较观察了Hayagar(HA)、Matingagar (MA)、Haycubeagar (HCA)、稻壳琼脂(RSA)、富营养Hayagar(IHA)以及沙保弱培养基 (SDA) 6种培养基的新生隐球菌交配试验效果。按 2周内产生阳性结果排列培养基的次序是HCA(95% )、RSA(95% )、HA(86% )、MA(38% )、SDA(38% )和IHA(2 4 % )。采用点状可以替代划线接种方法用于处理大批量标本并节省材料。此外 。Six media (MA:Mating agar,HA:Hay agar,HCA:Hay cube agar,RSA:Rice shell agar,IHA:additional nutrition in HA and SDA:Sabouraudia dextrose agar)were compared for their efficiencies of the mating test for Cryptococcus neoformans. The positive rates in 2\|week\|cultivation were 95% (HCA and RSA),86%(HA),38%(MA and SDA) and 24% (IHA).The streaking inoculation was equally replaced by the dot inoculation for the mating tests.However,the dot method saves umch more materials,and is suitable for testing in large number of isolates.Additionally,the precultivation of the tester and tested isolates in broth medium (YMPDB) could enhance the positive mating rates.

关 键 词：新生隐球菌 有性期 交配型分析 培养基 

分 类 号：Q93-3[生物学—微生物学]