应用线粒体DNA D-loop区遗传多样性分析云南4个少数民族的遗传关系  

Genetic Relationship Among Four Yunnan Populations Revealed by Sequence of mtDNA D-loop

作　　者：钱亚屏[1] 褚嘉佑[1] 初正韬[1] 卫灿东[1] 戴青[1] S Horai 

机构地区：[1]中国协和医科大学中国医学科学院医学生物学研究所,昆明650118 [2]National Institute of Genetics, Mishima 411

出　　处：《Acta Genetica Sinica》2001年第4期291-300,共10页

基　　金：国家自然科学基金重大项目!(39993420);美国中华医学基金CMB!(98-675);云南省青年基金!(1999C0025

摘　　要：对傣、佤、拉祜和藏族４个群体的９９名个体ｍｔＤＮＡ非编码区（Ｄ－ｌｏｏｐ）高变区I１６０４８～１６５６９及１～４１的５６３ｂｐ片段进行序列分析。计算了核酸多态度，并用Ｎｅｉｇｈｂｏｒ－Ｊｏｉｎｉｎｇ 法构建系统进化树，在进化树中，９９个ｍｔＤＮＡ序列分别聚在４个群中。所有在ＣＯ Ⅱ ／ｔＲＡＮ Ｌｙｓ 基因间序列存在９ｂｐ缺失的个体均聚在Ｃ１群中，Ｃ２群由１个佤族个体和４个藏族个体组成，Ｃ３群中除２个藏族个体外均为其他３个民族个体，４个群体的大部分个体聚在Ｃ４群。根据核酸多态度计算的净遗传距离重建的进化树显示，傣族、佤族和拉祜族的亲缘关系较接近，与藏族距离较远。结果表明遗传距离与他们的地理分布是非常一致的。而拉祜族与相传同为氐羌后裔并有相近语言的藏族跗距离却较远，这一结果提示这两个民族可能具有不同的起源。mtDNA D-loop noncoding region 16048～16569 and the following 1～41 (563bp) in 99 individuals of four Yunnan ethnic minorities (Dai, Wa, Lahu and Tibetan) were sequenced and then a phylogenetic tree was reconstructed by Neighbor-Joining method. T hese 99 mtDNA lineages were classified into 3 genotype groups in the tree. All lineages with 9 bp deletion in the COⅡ／tRANLys intergenic region were clustered in group Ⅰ, some individuals of Dai, Lahu, Wa and only 2 Tibetan individuals clustered in gr oup Ⅱ, individuals of all four populations were included in group Ⅲ. A phylogenetic tree of the four populations was constructed by NJ method on the basis of estimate of net genetic distance among them. Our results showed, the genetic distance among Dai , Wa and Lahu is very close, but far from Tibetan, their genetic distance is similar to their geographic distance. Although both as descendants of ancient Di-Qiang tribe in history and speaking similar language, Lahu and Tibetan are not closely related. T his result indicates that there are different origins of these two populations.

关 键 词：MTDNA D-LOOP 多态性 云南少数民族 遗传关系 遗传多样性 线粒体 

分 类 号：Q347[生物学—遗传学]