光对水稻非光合组织谷氨酰胺合成酶同工酶表达的影响(英文)  

Effect of Light on Expression of Glutamine Synthetase Isozymes in Non-photosynthetic Tissues of Rice Plants

作　　者：彭进[1] 李泽松[1] 林清华[1] 张楚富[1] PENG Shao-Bing BENNETT John 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,武汉430072 [2]International Rice Research Institute [3]International Rice esearch Institute

出　　处：《武汉植物学研究》2001年第1期31-34,共4页Journal of Wuhan Botanical Research

基　　金：The National Natural Science Foundation of China (No.39870 52 7) ;Cooperative Foundationof International Rice Research Institu

摘　　要：以前的研究表明 ,高等植物叶绿体谷氨酰胺合成酶 (GS2 )受光调节 ,但叶片胞液 GS(GS1 )和非光合作用组织中的 GS很少受光的影响。在本报道中 ,笔者运用 GS活性染色和Western blotting研究了光对非光合作用组织水稻根 GS同工酶表达的影响。在阳光的直接照射下以及在室内不同光照强度下 ,可以很清楚地观察到 GSra和 GSrb的活性带及其蛋白质带。但是 ,当用尼龙网挡住阳光的直接照射时 ,GSrb的活性带和蛋白质带消失 ;当阳光被尼龙网遮挡住后 ,其光强度仍然比室内光照强度大得多 ,表明光照强度不是影响 GSrb表达的主要因素。当分析生长在暗处以及生长在光 /暗转换下的水稻幼苗根 GS同工酶变化时 ,仍然可以观察到 GSrb的存在。在所有实验条件下 ,GSra都未发生明显变化。这些结果提示 。The previous studies showed that the expression of chloroplast GS2 is mediated by light but cytosolic GS1 and GS in non photosynthetic tissues of higher plants are little influenced.In the current report,we have investigated that GS isozymes in rice roots respond to light using GS activity staining and Western blotting.Both GSra and GSrb activity and their protein bands were detected at the illumination of sunlight and the incandescent lamp in the controlled environment chamber but GSrb activity and its protein bands were not observed under keeping out direct sunlight with the nylon net.The light intensity after the keeping out sunlight was higher than that of the incandescent lamp.This showed that light intensity was not a crucial factor for influencing GSrb expression.The activity and the protein bands of GSrb in rice roots were observed still in the light/dark transition and the dark seedlings.GSra activity was not remarkably changed at the light treatment.The results suggest that the effect of light on the expression of GSrb isoform in rice roots may be achieved through some unknown factor(s) resulted from the interaction of some spectra.

关 键 词：谷氨酰胺合成酶 同工酶 光 水稻 根 

分 类 号：S511.01[农业科学—作物学] Q945.11[生物学—植物学]