生物素标记核酸探针杂交检测灵敏度的比较研究  

STUDY ON THE DETECTING SENSITIVITY OF DNA HYBRIDIZED WITH BIOTINYLATED PROBES

作　　者：陈嘉勤[1] 方暨岚[2] 冯建生[2] 

机构地区：[1]湖南衡阳医学院心血管病研究所 [2]暨南大学医学院生化教研室

出　　处：《暨南大学学报（自然科学与医学版）》1991年第4期16-20,共5页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：用Biotin-11-dUTP进行缺口翻译制备生物素标记的人β-珠蛋白基因探针并用该探针进行了斑点杂交,硝酸纤维素膜印迹杂交及琼脂糖凝胶直接杂交方法学比较结果表明:经ABAP法显色后,上述三种杂交法中,DNA最低可检测量依次为5、10、50pg。A biotin-labeled probe of β-globin gene was prepared by a nick-translation procedure in which the labeled deoxynucleotide resoure was biotin-11-dUTP. Using the biotin-labeled probe of β-globin gene, a comparative study was made for dot-blot, southern blot and direct gel hybridization method. The results showed that the minimum detectable amounts of target DNA in samples were 5, 10, and 50pg respectively. Dot-blot hybridization method was shown to be the most sensititive.

关 键 词：生物素探针 核酸杂交 探针制备 DNA 

分 类 号：Q522[生物学—生物化学]