金黄色葡萄球菌性超抗原诱发银屑病发病机理探讨  

Study on the Pathogenesis of Psoriasis Induced by Staphylococcal Superantigen

作　　者：张峻岭[1] 张理涛[1] 陈学荣[2] 殷金珠[3] 

机构地区：[1]天津市长征医院皮肤科,300021 [2]北京大学第三医院皮肤科 [3]北京大学免疫室

出　　处：《中华皮肤科杂志》2001年第2期98-100,共3页Chinese Journal of Dermatology

摘　　要：目的探讨金黄色葡萄球菌性超抗原诱发银屑病的机制。方法 3H－TdR掺入法测定 细胞增殖反应，亚二倍体细胞含量测定，片段化 DNA分析，膜联蛋白 V (Annexin V)法测定 细胞凋亡，流式细胞仪检测细胞表面抗原表达。结果金黄色葡萄球菌肠毒素 B活化的淋巴细 胞培养上清液作用于角质形成细胞 48 h，可促进角质形成细胞增殖，诱导角质形成细胞表 达 HLA－DR和 Fas抗原；再次加入上清液继续作用 48 h，则诱导角质形成细胞凋亡（ P< 0.01）。结论金黄色葡萄球菌肠毒素 B作为超抗原活化 T细胞，使之释放细胞因子，后者使 角质形成细胞首先活化增殖，继而凋亡。Objective To investigate the pathogenesis of psoriasis induced by staphylococcal superantigen. Methods The proliferation responses of keratinocyte s were measured by 3H－TdR incorporation assay. Quantification of hypodiploid c ells, analysis of DNA fragmentation, and annexin V method were employed to detec t cell apoptosis. The expression of surface antigens on keratinocytes was determ ined by flow cytometric assay. Results The supernatants of peripheral blood lymp hocytes stimulated by staphylococcal enterotoxins B(SEB) could promote the proli feration of keratinocytes and induce the expression of HLA－DR and Fas antigens on keratinocytes after the first 48 h of treatment. Apoptosis of keratinocytes was induced after addition of the same supernatants（ P< 0.01） for another 48 h . Conclusions Cytokines released by T lymphocytes activated by SEB, a staphyloco ccal superantigen, may firstly stimulate the proliferation, and then induce apop tosis of kera tinocytes.

关 键 词：银屑病 金黄色葡萄球菌 超抗原 角蛋白细胞 细胞凋亡 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]