钙离子超载对低温保存人肝细胞的损伤作用  

Hypothermic Injury by Calcium Overload on Human Hepatocyte during Cold Storage

作　　者：李先亮[1] 刘永锋[1] 王凤山[1] 梁建[1] 何三光[1] 

机构地区：[1]中国医科大学第一临床学院普外一科暨器官移植科,沈阳110001

出　　处：《解剖科学进展》2001年第1期22-24,共3页Progress of Anatomical Sciences

摘　　要：目的　研究分离培养的成人肝细胞在低温保存状态下 (0～ 4℃ )的钙离子代谢特点和钙离子超载的损伤作用机理。方法　成人肝脏细胞分离和培养 ,用钙离子荧光探针检测保存液的钙离子浓度。结果　低温保存下 ,细胞内游离钙离子迅速升高 ,逐渐达到稳态水平 ,随细胞内钙离子的升高 ,细胞的存活率也逐渐下降 ,但在含0 .5 m MCa Cl2 的保存液组 ,保存效果明显好于其它两组 ;在含 1.5 m MCa Cl2 的保存液组 ,继发的钙离子超载峰值出现早而且要高于原发的钙超载峰。结论　低温保存可以引起成人肝细胞的钙超载 ,细胞外钙是细胞钙超载的主要来源 。Objective To explore the mechanism of hypothermic injury by calcium overload and the metabolism of calcium on human hepatocyte Results Intracellular free calcium increases rapidly and reaches to a hemeostasis slowly. As intracellular calcium increases, the cell survive rate decreases. But the effect of preservation in 0.5 mM CaCl 2 group is better than in other two groups. On 1.5mMCaCl 2 group, the peak of secondary calcium overload is much early and higher than that of primary one.Conclusion Hypothermic preservation may lead to human hepatocyte calcium overload, the extracellular calcium is a chief source of calcium overload, the secondary calcium overload is the main cause of ischemic reperfusion injury.

关 键 词：钙超载 肝细胞 低温保存 肝缺血 再灌注损伤 

分 类 号：R657.3[医药卫生—外科学] R318.52[医药卫生—临床医学]